التفاصيل البيبلوغرافية
| العنوان: |
Elucidating the Relations between Gut Bacterial Composition and the Plasma and Fecal Metabolomes of Antibiotic Treated Wistar Rats. |
| المؤلفون: |
Murali, Aishwarya, Giri, Varun, Cameron, Hunter James, Behr, Christina, Sperber, Saskia, Kamp, Hennicke, Walk, Tilmann, van Ravenzwaay, Bennard |
| المصدر: |
Microbiology Research; 2021, Vol. 12 Issue 1, p82-122, 41p |
| مصطلحات موضوعية: |
GUT microbiome, ANTIBIOTICS, DIET, METABOLOMICS, STATISTICAL correlation |
| مستخلص: |
The gut microbiome is vital to the health and development of an organism, specifically in determining the host response to a chemical (drug) administration. To understand this, we investigated the effects of six antibiotic (AB) treatments (Streptomycin sulfate, Roxithromycin, Sparfloxacin, Vancomycin, Clindamycin and Lincomycin hydrochloride) and diet restriction (-20%) on the gut microbiota in 28-day oral toxicity studies on Wistar rats. The fecal microbiota was determined using 16S rDNA marker gene sequencing. AB-class specific alterations were observed in the bacterial composition, whereas restriction in diet caused no observable difference. These changes associated well with the changes in the LC-MS/MS- and GC-MS-based metabolome profiles, particularly of feces and to a lesser extent of plasma. Particularly strong and AB-specific metabolic alterations were observed for bile acids in both plasma and feces matrices. Although AB-group-specific plasma metabolome changes were observed, weaker associations between fecal and plasma metabolome suggest a profound barrier between them. Numerous correlations between the bacterial families and the fecal metabolites were established, providing a holistic overview of the gut microbial functionality. Strong correlations were observed between microbiota and bile acids, lipids and fatty acids, amino acids and related metabolites. These microbiome-metabolome correlations promote understanding of the functionality of the microbiome for its host. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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