التفاصيل البيبلوغرافية
| العنوان: |
Force spectroscopy with electromagnetic tweezers. |
| المؤلفون: |
Piccolo, Joseph G., Méndez Harper, Joshua, McCalla, Derrica, Xu, Wenxuan, Miller, Sam, Doan, Jessie, Kovari, Dan, Dunlap, David, Finzi, Laura |
| المصدر: |
Journal of Applied Physics; 10/7/2021, Vol. 130 Issue 13, p1-13, 13p |
| مصطلحات موضوعية: |
ELECTROMAGNETIC forces, MAGNETIC tweezers, DEOXYRIBOZYMES, DNA helicases, PERMANENT magnets, MAGNETIC fields, OPTICAL tweezers |
| مستخلص: |
Force spectroscopy using magnetic tweezers (MTs) is a powerful method to probe the physical characteristics of single polymers. Typically, molecules are functionalized for specific attachment to a glass surface at one end and a micrometer-scale paramagnetic bead at the other end. By applying an external magnetic field, multiple molecules can be stretched and twisted simultaneously without exposure to potentially damaging radiation. The majority of MTs utilize mobile, permanent magnets to produce forces on the beads (and the molecule under test). However, translating and rotating the permanent magnets may require expensive precision actuators, limit the rate at which force can be changed, and may induce vibrations that disturb tether dynamics and bead tracking. Alternatively, the magnetic field can be produced with an electromagnet, which allows fast force modulation and eliminates motor-associated vibration. Here, we describe a low-cost quadrapolar electromagnetic tweezer design capable of manipulating DNA-tethered MyOne paramagnetic beads with forces as high as 15 pN. The solid-state nature of the generated B-field modulated along two axes is convenient for accessing the range of forces and torques relevant for studying the activity of DNA motor enzymes like polymerases and helicases. Our design specifically leverages technology available at an increasing number of university maker spaces and student-run machine shops. Thus, it is an accessible tool for undergraduate education that is applicable to a wide range of biophysical research questions. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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