التفاصيل البيبلوغرافية
| العنوان: |
Trypanosoma brucei gambiense-iELISA: A Promising New Test for the Post-Elimination Monitoring of Human African Trypanosomiasis. |
| المؤلفون: |
Geerts, Manon, Reet, Nick Van, Leyten, Sander, Berghmans, Raf, Rock, Kat S, Coetzer, Theresa H T, Eyssen, Lauren E-A, Büscher, Philippe |
| المصدر: |
Clinical Infectious Diseases; 11/1/2021, Vol. 73 Issue 9, pe2477-e2483, 7p |
| مصطلحات موضوعية: |
PROTOZOA, CONFIDENCE intervals, MONOCLONAL antibodies, PATIENT monitoring, TRYPANOSOMIASIS, ENZYME-linked immunosorbent assay, GLYCOPROTEINS, DESCRIPTIVE statistics |
| مستخلص: |
Background The World Health Organization targeted Trypanosoma brucei gambiense human African trypanosomiasis (g HAT) for elimination as a public health problem and for elimination of transmission. To measure g HAT elimination success with prevalences close to zero, highly specific diagnostics are necessary. Such a test exists in the form of an antibody-mediated complement lysis test, the trypanolysis test, but biosafety issues and technological requirements prevent its large-scale use. We developed an inhibition ELISA with high specificity and sensitivity that is applicable in regional laboratories in g HAT endemic countries. Methods The T. b. gambiense inhibition ELISA (g -iELISA) is based on the principle that binding of monoclonal antibodies to specific epitopes of T. b. gambiense surface glycoproteins can be inhibited by circulating antibodies of g HAT patients directed against the same epitopes. Using trypanolysis as reference test, the diagnostic accuracy of the g -iELISA was evaluated on plasma samples from 739 g HAT patients and 619 endemic controls and on dried blood spots prepared with plasma of 95 g HAT and 37 endemic controls. Results Overall sensitivity and specificity on plasma were, respectively, 98.0% (95% CI 96.7–98.9) and 99.5% (95% CI 98.6–99.9). With dried blood spots, sensitivity was 92.6% (95% CI 85.4–97.0), and specificity was 100% (95% CI 90.5–100.0). The g -iELISA is stable for at least 8 months when stored at 2–8°C. Conclusion The g -iELISA might largely replace trypanolysis for monitoring g HAT elimination and for postelimination surveillance. The g -iELISA kit is available for evaluation in reference laboratories in endemic countries. [ABSTRACT FROM AUTHOR] |
|
Copyright of Clinical Infectious Diseases is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder