التفاصيل البيبلوغرافية
| العنوان: |
Vascular KATP channel structural dynamics reveal regulatory mechanism by Mg-nucleotides. |
| المؤلفون: |
Min Woo Sung, Zhongying Yang, Driggers, Camden M., Patton, Bruce L., Mostofian, Barmak, Russo, John D, Zuckerman, Daniel M., Show-Ling Shyng |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 11/2/2021, Vol. 118 Issue 44, p1-12, 12p |
| مصطلحات موضوعية: |
STRUCTURAL dynamics, MOLECULAR dynamics, SMOOTH muscle, ELECTRON microscopy, ATP-binding cassette transporters |
| مستخلص: |
Vascular tone is dependent on smooth muscle KATP channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantú syndrome. Unique among KATP isoforms, they lack spontaneous activity and require Mg-nucleotides for activation. Structural mechanisms underlying these properties are unknown. Here, we determined cryogenic electron microscopy structures of vascular KATP channels bound to inhibitory ATP and glibenclamide, which differ informatively from similarly determined pancreatic KATP channel isoform (Kir6.2/SUR1). Unlike SUR1, SUR2B subunits adopt distinct rotational “propeller” and “quatrefoil” geometries surrounding their Kir6.1 core. The glutamate/aspartate-rich linker connecting the two halves of the SUR-ABC core is observed in a quatrefoil-like conformation. Molecular dynamics simulations reveal MgADP-dependent dynamic tripartite interactions between this linker, SUR2B, and Kir6.1. The structures captured implicate a progression of intermediate states between MgADP-free inactivated, and MgADP-bound activated conformations wherein the glutamate/aspartate-rich linker participates as mobile autoinhibitory domain, suggesting a conformational pathway toward KATP channel activation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
