التفاصيل البيبلوغرافية
| العنوان: |
Influence of electron beam irradiation on extracellular matrix of the human allogeneic skin grafts. |
| المؤلفون: |
Łabuś, Wojciech, Kitala, Diana, Klama‐Baryła, Agnieszka, Szapski, Michał, Kraut, Małgorzata, Smętek, Wojciech, Glik, Justyna, Kucharzewski, Marek, Rojczyk, Ewa, Utrata‐Wesołek, Alicja, Trzebicka, Barbara, Szeluga, Urszula, Sobota, Michał, Poloczek, Ryszard, Kamiński, Artur |
| المصدر: |
Journal of Biomedical Materials Research, Part B: Applied Biomaterials; Mar2022, Vol. 110 Issue 3, p547-563, 17p |
| مصطلحات موضوعية: |
SKIN grafting, ELECTRON beams, EXTRACELLULAR matrix, RADIATION sterilization, IRRADIATION |
| مستخلص: |
The nonviable allogeneic human skin grafts might be considered as the most suitable skin substitutes in the treatment of extensive and deep burns. However, in accordance to biological security such grafts require the final sterilization prior to clinical application. The aim of the study was to verify the influence of electron beam irradiation of three selected doses: 18, 25, and 35 kGy on the extracellular matrix of human skin. Prior to sterilization, the microbiological tests were conducted and revealed contamination in all examined cases. Individual groups were subjected to single electron beam radiation sterilization at proposed doses and then subjected to microbiological tests again. The results of microbiological testing performed for all irradiation doses used were negative. Only in the control group was a growth of microorganisms observed. The FTIR spectrometry tests were conducted followed by the histological evaluation and mechanical tests. In addition, cost analysis of radiation sterilization of individual doses was performed. The results of spectroscopic analysis, mechanical tests, and histological staining showed no significant changes in composition and characteristics of tested tissues after their irradiation, in comparison to control samples. The cost analysis has shown that irradiation with 18 kGy is the most cost‐effective and 35 kGy is the least favorable. However, according to biological risk reduction, the recommended sterilization dose is 35 kGy, despite the higher price compared to the other doses tested. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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