التفاصيل البيبلوغرافية
| العنوان: |
Upper limb arthromyodynia can be treated with Notopterygium by down-regulating P2X3 to mediate PKC-induced inflammatory response. |
| المؤلفون: |
Yingzheng Wang, Ce Yang, Cuiping Zhu, Qianqian Zhao, Xuehua Lu, Xiaoyu Su, Yinghao Wang |
| المصدر: |
Journal of Chinese Pharmaceutical Sciences; Mar2022, Vol. 31 Issue 3, p163-175, 13p |
| مصطلحات موضوعية: |
INFLAMMATION, SPRAGUE Dawley rats, CHINESE medicine, WESTERN immunoblotting, ETHYL acetate |
| مستخلص: |
Rheumatoid arthritis (RA) is one of the most common refractory diseases in the world, and traditional Chinese medicine Notopterygium (NE) has been used in the treatment of upper limb pain for a long time. NE can significantly reduce the expression of inflammatory pain target P2X3 receptor in rats with upper-limb arthritis. To verify the relationship between the mechanism of NE for "upper limb paralysis" and the P2X3 receptor-mediated PKC inflammatory response pathway, UPLC was taken to measure the exact medicinal substance of ethyl acetate from NE. Sprague Dawley rats were randomly divided into a blank group, a model group, a live-action group, and a positive group. The joint cavity was removed after 21 d. Moreover, a model group, a live group, and a positive group were also set up with RA-FLS cells in our in vitro study. The expressions of P2X3 and PKC inflammation pathway indicators were detected by Western blotting analysis. A P2X3 inhibitor (A-317491) acted on RA-FLS cells, and a model group and a positive group were set. Then the protein expression of PKC was detected. NE reduced the expressions of P2X3, Rab7, PKC, and NF-κB at the protein level in both systems. NE and P2X3 receptor antagonists reduced the expressions of key proteins in the PKC pathway in RA-FLS cells to similar extents, and their effects were not additive. NE could effectively improve the "forelimb pain" of RA rats, with a mechanism closely related to the P2X3/Rab7/PKC/NF-κB pathway. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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