التفاصيل البيبلوغرافية
| العنوان: |
Profile of Innate Immunity in Gilthead Seabream Larvae Reflects Mortality upon Betanodavirus Reassortant Infection and Replication. |
| المؤلفون: |
García-Álvarez, Miguel Ángel, Arizcun, Marta, Chaves-Pozo, Elena, Cuesta, Alberto |
| المصدر: |
International Journal of Molecular Sciences; May2022, Vol. 23 Issue 9, p5092-5092, 16p |
| مصطلحات موضوعية: |
SPARUS aurata, NATURAL immunity, CELL-mediated cytotoxicity, PEPTIDES, VIRAL replication, LARVAE, EGG incubation |
| مستخلص: |
Historically, gilthead seabream (Sparus aurata) has been considered a fish species resistant to nervous necrosis virus (NNV) disease. Nevertheless, mortality in seabream hatcheries, associated with typical clinical signs of the viral encephalopathy and retinopathy (VER) disease has been confirmed to be caused by RGNNV/SJNNV reassortants. Because of this, seabream larvae at 37 and 86 days post-hatching (dph) were infected by immersion with RGNNV/SJNNV and SJNNV/RGNNV reassortants under laboratory conditions, and mortality, viral replication and immunity were evaluated. Our results show that gilthead seabream larvae, mainly those at 37 dph, are susceptible to infection with both NNV reassortant genotypes, with the highest impact from the RGNNV/SJNNV reassortant. In addition, viral replication occurs at both ages (37 and 86 dph) but the recovery of infective particles was only confirmed in 37 dph larvae,; this value was also highest with the RGNNV/SJNNV reassortant. Larvae immunity, including the expression of antiviral, inflammatory and cell-mediated cytotoxicity genes, was affected by NNV infection. Levels of the natural killer lysin (Nkl) peptide were increased in SJNNV/RGNNV-infected larvae of 37 dph, though hepcidin was not. Our results demonstrate that the seabream larvae are susceptible to both NNV reassortants, though mainly to RGNNV/SJNNV, in an age-dependent manner. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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