التفاصيل البيبلوغرافية
العنوان: |
SUSCEPTIBILIDAD ANTIMICROBIANA DE Staphylococcus aureus AISLADOS DE VACAS HOLSTEIN PORTADORAS DE MASTITIS SUBCLÍNICA EN LA PROVINCIA DE BUENOS AIRES, ARGENTINA. (Spanish) |
Alternate Title: |
ANTIMICROBIAL SUSCEPTIBILITY OF Staphylococcus aureus ISOLATED FROM HOLSTEIN COWS WITH SUBCLINICAL MASTITIS IN THE PROVINCE OF BUENOS AIRES, ARGENTINA. (English) |
المؤلفون: |
F., Aliverti, M., Lucas, D., Buldain, L., Marchetti, N., Mestorino |
المصدر: |
Veterinaria Cuyana; 2021, Vol. 15, p5-14, 10p |
Abstract (English): |
Bovine mastitis is the disease with the greatest economic impact on dairy farms worldwide. Staphylococcus aureus is one of the main culprits, capable of surviving intracellularly and developing different resistance mechanisms. The objective of the present study was to determine the susceptibility to antimicrobials of S. aureus strains isolated from milk samples obtained from Holstein cows in production with subclinical mastitis. A total of 44 Staphylococcus aureus strains were analyzed by disk diffusion on agar (DDA). Oxacillin, cefoxitin, clindamycin, erythromycin, rifampicin, rifaximin, and cefacetril/rifaximin discs were used. 2.27% strains were resistant to clindamycin and erythromycin. 95.45% presented sensitivity to all the antibiotics tested. 17 colonies were isolated from the inhibition halos produced by oxacillin and rifampicin, of which 10 were confirmed as S. aureus strains. From these, a second DDA test was performed. Antibiotic resistance to erythromycin was 20%, and 70% for rifampicin and rifaximin. Knowing the susceptibility of the microorganisms that produce mastitis is essential to achieve effective antibiotic therapy; thus avoiding multi-resistance due to the indiscriminate use of antibiotics in the dairy farms. [ABSTRACT FROM AUTHOR] |
Abstract (Spanish): |
La mastitis bovina es la enfermedad con mayor impacto económico en las explotaciones lecheras a nivel mundial. Staphylococcus aureus es uno de los principales responsables, capaz de sobrevivir a nivel intracelular y desarrollar diferentes mecanismos de resistencia. El objetivo del presente estudio fue determinar la susceptibilidad a los antimicrobianos de cepas de S. aureus aisladas de muestras de leche obtenidas de vacas Holstein en producción y portadoras de mastitis subclínica. Un total de 44 cepas de Staphylococcus aureus fueron analizadas mediante difusión de disco en agar (DDA). Se utilizaron discos de oxacilina, cefoxitina, clindamicina, eritromicina, rifampicina, rifaximina y cefacetrilo/rifaximina. Se encontró un 2,27 % de cepas resistentes a clindamicina y eritromicina. El 95,45 % presentó sensibilidad a todos los antibióticos ensayados. Se aislaron 17 colonias a partir de los halos de inhibición producidos por oxacilina y rifampicina, de las cuales 10 fueron confirmadas como cepas de S. aureus. A partir de estas, se realizó una segunda prueba de DDA. La resistencia antibiótica a eritromicina fue de 20%, y 70% para rifampicina y rifaximina. Conocer la susceptibilidad de los microorganismos causantes de mastitis es fundamental para lograr una terapia antibiótica efectiva; evitando así multirresistencia por el uso indiscriminado de antibióticos en el tambo.f [ABSTRACT FROM AUTHOR] |
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قاعدة البيانات: |
Complementary Index |