| مستخلص: |
Mucuna pruriens var. utilis (Wall. ex Wight) Baker ex Burck has been used as a traditional medicine for a long time to treat several diseases such as malaria, cancer, diabetes, Parkinson's disease, anxiety disorder and to improve sexual performance. This research investigated phytochemical constituents, anxiety and stress relieving properties, and antidiabetic activities of seed extract from M. pruriens (MPSE) extracted with 95% ethanol. Analysis using GC-MS revealed the presence of 48 chemical compounds with 9 main compounds including 5-Hydroxymethylfurfural, Dodecanamide, N-(2-hydroxyethyl)-, 9,12-Octadecadienoic acid, (Z, Z)-, n-Hexadecanoic acid, endo-Borneol, 9,12-Octadecadienoic acid, ethyl ester, Hexadecanoic acid, ethyl ester, Octadecanoic acid and 9-Octadecenoic acid, and another 39 trace compounds in the extract. Evaluation using light/dark task, hole board and force plate actimeter (FPA) revealed that the number of entries into the chamber, number of rear in dark chamber, time spent in light chamber and the number of entries to the hole board of the 400 mg/kg body weight, MPSE treated mice were significantly (p<0.05) higher than those of the controls, whilst the latency to enter the light chamber was less than that of the controls. In addition, the sleeping time of the MPSE treated mice was significantly (p<0.05) longer than that of the controls. Furthermore, MPSE exhibited an inhibitory effect on α-glucosidase activity with IC50 of 0.38±0.70 mg/mL, which was better than Acarbose (IC50 of 1.57±0.23 mg/mL). The results indicate that MPSE has anxiety and stress relieving properties and also antidiabetic activities. The endo-Bomeol and Octadecanoic acid were responsible for anxiety and stress relieving activity, and 9, 12-Octadecadienoic acid, (Z, Z)- and n-Hexadecanoic acid were responsible for antidiabetic activity. The results obtained also confirmed the traditional used of M. pruriens for relieving anxiety disorder and stress, and the treatment of diabetes mellitus. [ABSTRACT FROM AUTHOR] |
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