التفاصيل البيبلوغرافية
| العنوان: |
EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes. |
| المؤلفون: |
Koester, Anna M., Geiser, Angéline, Laidlaw, Kamilla M. E., Morris, Silke, Cutiongco, Marie F. A., Stirrat, Laura, Gadegaard, Nikolaj, Boles, Eckhard, Black, Hannah L., Bryant, Nia J., Gould, Gwyn W. |
| المصدر: |
Bioscience Reports; Jul2022, Vol. 42 Issue 7, p1-13, 13p |
| مصطلحات موضوعية: |
FAT cells, GLUCOSE, GLUCOSE transporters, SCAFFOLD proteins, INSULIN receptors, CELL membranes, INSULIN, GLUCOSE-regulated proteins |
| مستخلص: |
Insulin stimulates glucose transport in muscle and adipocytes. This is achieved by regulated delivery of intracellular glucose transporter (GLUT4)-containing vesicles to the plasmamembrane where they dock and fuse, resulting in increased cell surface GLUT4 levels. Recent work identified a potential further regulatory step, in which insulin increases the dispersal of GLUT4 in the plasmamembrane away from the sites of vesicle fusion. EFR3 is a scaffold protein that facilitates localization of phosphatidylinositol 4-kinase type IIIα to the cell surface. Here we show that knockdown of EFR3 or phosphatidylinositol 4-kinase type IIIα impairs insulin-stimulated glucose transport in adipocytes. Using direct stochastic reconstruction microscopy, we also show that EFR3 knockdown impairs insulin stimulated GLUT4 dispersal in the plasma membrane. We propose that EFR3 plays a previously unidentified role in controlling insulin-stimulated glucose transport by facilitating dispersal of GLUT4 within the plasma membrane. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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