التفاصيل البيبلوغرافية
العنوان: |
ANALYSIS OF CRUDE RICIN FROM Ricinus communis ORIGINATED FROM NGANJUK, EAST JAVA, INDONESIA, USING LIQUID CHROMATOGRAPHY, COLUMN LIQUID CHROMATOGRAPHY, AND FAST PROTEIN LIQUID CHROMATOGRAPHY (FPLC). |
المؤلفون: |
Herawati, I. E., Lesmana, R., Levita, J., Subarnas, A. |
المصدر: |
Rasayan Journal of Chemistry; Jan-Mar2022, Vol. 15 Issue 1, p204-209, 6p |
مصطلحات موضوعية: |
RICIN, LIQUID chromatography, CASTOR oil plant, COLUMN chromatography, POISONS, CHEMICAL properties |
مصطلحات جغرافية: |
INDONESIA, PAPUA (Indonesia) |
مستخلص: |
Ricinus communis L. (Euphorbiaceae), the local name jarak kepyar, is a tropical plant that is widely planted in East Java, Indonesia. The leaves and seeds of this plant have been traditionally utilized to cure liver disease, stomach disorders, inflammation, fever, headache, etc. Ricin, one of the most toxic substances known isolated from R. communis L. seeds, is a heterodimeric two-domain polypeptide protein that includes chain A (30 kDa) and a slightly bigger chain B (35 kDa). The technique to distinguish and separate ricin is not much reported. In this study, we analyzed the crude ricin extracted from R. communis L. seeds originated from Nganjuk, East Java, Indonesia. The techniques used were (1) liquid chromatography (LC); (2) column liquid chromatography (CLC); and (2) fast protein liquid chromatography (FPLC), followed by SDS-PAGE. Results show that all techniques positively confirm the presence of ricin protein indicated by a doublet peak in all chromatograms. This study might contribute to understanding the biological and chemical properties of the ricin protein of R. communis L. seeds. [ABSTRACT FROM AUTHOR] |
|
Copyright of Rasayan Journal of Chemistry is the property of Rasayan Journal of Chemistry and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
قاعدة البيانات: |
Complementary Index |