التفاصيل البيبلوغرافية
| العنوان: |
SATB1 ensures appropriate transcriptional programs within naïve CD8+ T cells. |
| المؤلفون: |
Nüssing, Simone, Miosge, Lisa A, Lee, Kah, Olshansky, Moshe, Barugahare, Adele, Roots, Carla M, Sontani, Yovina, Day, E Bridie, Koutsakos, Marios, Kedzierska, Katherine, Goodnow, Christopher C, Russ, Brendan E, Daley, Stephen R, Turner, Stephen J |
| المصدر: |
Immunology & Cell Biology; Sep2022, Vol. 100 Issue 8, p636-652, 17p |
| مصطلحات موضوعية: |
CHROMATIN, CD8 antigen, T cells, INFLUENZA A virus |
| مستخلص: |
Special AT‐binding protein 1 (SATB1) is a chromatin‐binding protein that has been shown to be a key regulator of T‐cell development and CD4+ T‐cell fate decisions and function. The underlying function for SATB1 in peripheral CD8+ T‐cell differentiation processes is largely unknown. To address this, we examined SATB1‐binding patterns in naïve and effector CD8+ T cells demonstrating that SATB1 binds to noncoding regulatory elements linked to T‐cell lineage–specific gene programs, particularly in naïve CD8+ T cells. We then assessed SATB1 function using N‐ethyl‐N‐nitrosourea‐mutant mice that exhibit a point mutation in the SATB1 DNA‐binding domain (termed Satb1m1Anu/m1Anu). Satb1m1Anu/m1Anu mice exhibit diminished SATB1‐binding, naïve, Satb1m1Anu/m1Anu CD8+ T cells exhibiting transcriptional and phenotypic characteristics reminiscent of effector T cells. Upon activation, the transcriptional signatures of Satb1m1Anu/m1Anu and wild‐type effector CD8+ T cells converged. While there were no overt differences, primary respiratory infection of Satb1m1Anu/m1Anu mice with influenza A virus (IAV) resulted in a decreased proportion and number of IAV‐specific CD8+ effector T cells recruited to the infected lung when compared with wild‐type mice. Together, these data suggest that SATB1 has a major role in an appropriate transcriptional state within naïve CD8+ T cells and ensures appropriate CD8+ T‐cell effector gene expression upon activation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
