التفاصيل البيبلوغرافية
| العنوان: |
Microglia activation and temporal changes in rat model of trigeminal neuralgia. (English) |
| المؤلفون: |
Lu Yanzhu, Zhang Jingqi, Lai Wenli |
| المصدر: |
West China Journal of Stomatology; Dec2022, Vol. 40 Issue 6, p638-644, 7p |
| مصطلحات موضوعية: |
TRIGEMINAL neuralgia, ANIMAL disease models, PAIN threshold, MICROGLIA, TRIGEMINAL nerve, NERVE grafting |
| مستخلص: |
Objective This study aimed to investigate whether the microglia in the spinal trigeminal nucleus caudal part (Sp5C) were activated in a rat model of trigeminal neuralgia and to explore whether the activation level of microglia is consistent with maxillofacial pain level. Methods Chronic constriction injury of trigeminal nerve (CCI) was induced by partial ligation of infraorbital nerve (IoN) in rats. The behavioral change of rats observed at D1, D5, D10, D15, and D30 days post-surgery and the change of pain threshold were detected with electronic Von Frey filaments served as an evaluation index of maxillofacial pain. Weight change was measured by weighing. Ionized calcium binding adaptor molecule- 1 (Iba-1) expression level of Sp5C at each time point was detected, and three microglia morphological categories were analyzed by immunohistochemical staining. Results The changes of behavioral and pain threshold suggested the maxillofacial pain level first increased and then decreased post-surgery in the IoN-CCI group. Both the expressions of Iba-1 and proportion of ameboid morphology in ipsilateral Sp5C increased from D1 and reached their peaks in D10 and D5, respectively. Then, they recovered nearly to the same level with contralateral Sp5C on D30. This trend was consis consistent with the maxillofacial change. Conclusion The model of trigeminal neuralgia in rats constructed by partial ligation of infraorbital nerve can induce the activation of microglia in Sp5C, and the activation level is consistent with maxillofacial pain, which reached its peak at around D10 post-surgery. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
