التفاصيل البيبلوغرافية
| العنوان: |
Precise epigenomic editing with a SunTag-based modular epigenetic toolkit. |
| المؤلفون: |
Guhathakurta, Subhrangshu, Adams, Levi, Jeong, Inhye, Sivakumar, Anishaa, Mingyu Cha, Fiadeiro, Mariana Bernardo, Hu, Haiyan Nancy, Yoon-Seong Kim |
| المصدر: |
Epigenetics; Dec2022, Vol. 17 Issue 13, p2075-2081, 7p |
| مصطلحات موضوعية: |
EPIGENETICS, PROMOTERS (Genetics), GENE expression |
| مستخلص: |
Epigenetic regulation is a crucial factor controlling gene expression. Here, we report our CRISPR/dCas9-based modular epigenetic toolkit that enables gene-specific modulation of epigenetic architecture. By modifying the SunTag framework of dCas9 tagged with five GCN4 moieties, each epigenetic writer is bound to scFv and target-specific sgRNA, and this system is able to modify multiple epigenetic marks in a target-specific manner. We successfully demonstrated that this system is efficient in modifying individual histone post-translational modifications. We display its utility as a tool to understand the contributions of specific histone marks on gene expression by screening a large promoter region and identifying differential outcomes with high base-pair resolution. This epigenetic toolkit can be easily altered with a large variety of epigenetic effectors and is a useful tool for researchers to use in understanding gene-specific epigenetic changes and their relation to gene expression. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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