التفاصيل البيبلوغرافية
| العنوان: |
Colocalization Analysis of Cytoplasmic Actin Isoforms Distribution in Endothelial Cells. |
| المؤلفون: |
Shakhov, Anton S., Kovaleva, Polina A., Churkina, Alexandra S., Kireev, Igor I., Alieva, Irina B. |
| المصدر: |
Biomedicines; Dec2022, Vol. 10 Issue 12, p3194, 12p |
| مصطلحات موضوعية: |
ENDOTHELIAL cells, ACTIN, CELL anatomy, CYTOSKELETON, AMINO acid sequence |
| مستخلص: |
Actin cytoskeleton is an essential component of living cells and plays a decisive role in many cellular processes. In mammals, β- and γ-actin are cytoplasmic actin isoforms in non-muscle cells. Despite minor differences in the amino acid sequence, β- and γ-actin localize in different cell structures and perform different functions. While cytoplasmic β-actin is involved in many intracellular processes including cell contraction, γ-actin is responsible for cell mobility and promotes tumor transformation. Numerous studies demonstrate that β- and γ-actin are spatially separated in the cytoplasm of fibroblasts and epithelial cells; this separation is functionally determined. The spatial location of β/γ-actin in endothelial cells is still a subject for discussion. Using super-resolution microscopy, we investigated the β/γ-actin colocalization in endotheliocytes and showed that the β/γ-actin colocalization degree varies widely between different parts of the marginal regions and near the cell nucleus. In the basal cytoplasm, β-actin predominates, while the ratio of isoforms evens out as it moves to the apical cytoplasm. Thus, our colocalization analysis suggests that β- and γ-actin are segregated in the endotheliocyte cytoplasm. The segregation is greatly enhanced during cell lamella activation in the nocodazole-induced endothelial barrier dysfunction, reflecting a different functional role of cytoplasmic actin isoforms in endothelial cells. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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