التفاصيل البيبلوغرافية
| العنوان: |
In Skeletal Muscle Fibers, Protein Kinase Subunit CSNK2A1/CK2α Is Required for Proper Muscle Homeostasis and Structure and Function of Neuromuscular Junctions. |
| المؤلفون: |
Merholz, Mira, Jian, Yongzhi, Wimberg, Johannes, Gessler, Lea, Hashemolhosseini, Said |
| المصدر: |
Cells (2073-4409); Dec2022, Vol. 11 Issue 24, p3962, 17p |
| مصطلحات موضوعية: |
MYONEURAL junction, SKELETAL muscle, PROTEIN kinases, HOMEOSTASIS, NEUROMUSCULAR transmission, PROTEIN kinase CK2, MUSCLE weakness, CYTOCHROME oxidase |
| مستخلص: |
CSNK2 tetrameric holoenzyme is composed of two subunits with catalytic activity (CSNK2A1 and/or CSNK2A2) and two regulatory subunits (CSNK2B) and is involved in skeletal muscle homeostasis. Up-to-date, constitutive Csnk2a2 knockout mice demonstrated mild regenerative impairments in skeletal muscles, while conditional Csnk2b mice were linked to muscle weakness, impaired neuromuscular transmission, and metabolic and autophagic compromises. Here, for the first time, skeletal muscle-specific conditional Csnk2a1 mice were generated and characterized. The ablation of Csnk2a1 expression was ensured using a human skeletal actin-driven Cre reporter. In comparison with control mice, first, conditional knockout of CSNK2A1 resulted in age-dependent reduced grip strength. Muscle weakness was accompanied by impaired neuromuscular transmission. Second, the protein amount of other CSNK2 subunits was aberrantly changed. Third, the number of central nuclei in muscle fibers indicative of regeneration increased. Fourth, oxidative metabolism was impaired, reflected by an increase in cytochrome oxidase and accumulation of mitochondrial enzyme activity underneath the sarcolemma. Fifth, autophagic processes were stimulated. Sixth, NMJs were fragmented and accompanied by increased synaptic gene expression levels. Altogether, knockout of Csnk2a1 or Csnk2b results in diverse impairments of skeletal muscle biology. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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