التفاصيل البيبلوغرافية
| العنوان: |
Stimulation of Xenopus oocyte Na+,K+ATPase by the serum and glucocorticoid-dependent kinase sgk1. |
| المؤلفون: |
Setiawan, Iwan, Henke, Guido, Feng, Yuxi, Böhmer, Christoph, Vasilets, Larisa A., Schwarz, Wolfgang, Lang, Florian |
| المصدر: |
Pflügers Archiv: European Journal of Physiology; Jun2002, Vol. 444 Issue 3, p426-431, 6p |
| مصطلحات موضوعية: |
SERUM, GLUCOCORTICOIDS, XENOPUS, EPITHELIAL cells, ALDOSTERONE, VENTILATION |
| مستخلص: |
The serum and glucocorticoid-dependent kinase-1 (sgk1) is expressed in a wide variety of tissues including renal epithelial cells. As it is up-regulated by aldosterone, it is considered to participate in the regulation of renal Na+ reabsorption. Indeed, co-expression of sgk1 with the renal epithelial Na+ channel (ENaC) augments Na+ channel activity. The aim of the present study was to examine possible effects of sgk1 on Na+/K+-ATPase activity. To this end dual-electrode voltage-clamp experiments were performed in Xenopus oocytes expressing the active kinase S422Dsgk1 or the inactive mutant K127Nsgk1. Na+/K+-ATPase activity was estimated from the hyperpolarization (ΔVm) and the outwardly-directed current (IP) created by addition of extracellular K+ in the presence of K+ channel blocker Ba2+. Both ΔVm and IP were significantly larger in oocytes expressing S422Dsgk1 than in those expressing K127Nsgk1 or having been injected with water. IP was fully inhibited by ouabain. Ion-selective microelectrodes showed that the stimulation of pump current was not the result of altered cytosolic Na+ activity or pH. The present results thus point to an additional action of sgk1 that may participate in the regulation of renal tubular Na+ transport. Moreover, sgk1 may be involved in the regulation of Na+/K+-ATPase in extrarenal tissues. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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