التفاصيل البيبلوغرافية
| العنوان: |
Keragaman Gen BG1 sebagai Kandidat Gen Penciri Ketahanan Penyakit pada Calon Galur Ayam IPB-D2. (Indonesian) |
| Alternate Title: |
Polymorphisms of the BG1 Gene as a Candidate for Disease Resistance Characterizing Genes in IPB-D2 Prospective Chicken Lines. (English) |
| المؤلفون: |
Miraj, N. N., Sumantri, C., Murtini, S., Ulupi, N. |
| المصدر: |
Journal of Animal Production & Processing Technology / JurnalIlmu Produksi dan Teknologi Hasil Peternakan; okt2022, Vol. 10 Issue 3, p144-151, 8p |
| Abstract (English): |
The BG1 gene is a disease resistance candidate gene in chicken, which is located in the MHC region. The mechanism of the BG1 gene is known as an inhibitor of T lymphocyte cell activation. This study aims to identify the diversity of the SNP gene BG1 in the IPB-D2 chicken line. IPB-D2 chicken is a candidate for a new line from a cross from IPB-D1 chicken, which was selected for high body resistance and good productivity. A total of 27 DNA samples of IPB-D2 (G0) chickens and 58 samples of IPB-D2 (G2) chickens were sequenced to identify the diversity of BG1 genes. The study found 6 point mutations (g.4489 G>T, g.4712 C>T, g.4741 T>C, g.4747 T>C, g.4749 T>C, g.4761 G>A) and deletion of 2 nucleotide bases g.4758 - g.4759 delAT. The combination of 6 SNPs forms 4 haplotypes. The SNPs that changed the amino acid composition were SNP g.4489 G>T cys> phen, g.4712 C>T gln>codon stop, g.4749 T>C val>ala and g.4761 G> A gly> glu. All mutations are classified as polymorphic, low heterozygosity value, and not in Hardy-Weinberg equilibrium. [ABSTRACT FROM AUTHOR] |
| Abstract (Indonesian): |
Gen BG1 merupakan gen kandidat ketahanan penyakit pada ayam yang terletak di wilayah MHC. Mekanisme gen BG1 diketahui sebagai penghambat aktivasi sel limfosit T. Penelitian ini bertujuan mengidentifikasi keragaman SNP gen BG1 pada ayam IPB-D2. Ayam IPB-D2 merupakan calon galur baru hasil persilangan dari ayam IPB-D1 yang diseleksi terhadap sifat ketahanan tubuh yang tinggi serta memiliki produktivitas yang baik. Sejumlah 27 sampel DNA ayam IPB-D2 (G0) dan 58 sampel ayam IPB-D2 (G2) di sekuensing untuk mengidentifikasi keragaman gen BG1. Hasil penelitian ditemukan 6 titik mutasi (g.4489 G>T, 4712 C>T, g.4741 T>C, g.4747 T>C, g.4749 T>C, g.4761 G>A) dan delesi 2 basa nukleotida g.4758 - g.4759 delAT. Kombinasi 6 SNP membentuk 4 haplotipe. SNP yang mengubah komposisi asam amino terdapat pada SNP g.4489 G>T cys>phen, g.4712 C>T gln>codon stop, g.4749 T>C val>ala dan g.4761 G>A gly>glu. Semua mutasi diklasifikasikan polimorfik, nilai heterozigositas rendah dan tidak berada dalam kesetimbangan Hardy-Weinberg. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
