التفاصيل البيبلوغرافية
| العنوان: |
3D bioprinting of an implantable xeno-free vascularized human skin graft. |
| المؤلفون: |
Baltazar, Tania, Bo Jiang, Moncayo, Alejandra, Merola, Jonathan, Albanna, Mohammad Z., Saltzman, W. Mark, Pober, Jordan S. |
| المصدر: |
Bioengineering & Translational Medicine; Jan2023, Vol. 8 Issue 1, p1-16, 16p |
| مصطلحات موضوعية: |
BIOPRINTING, SKIN grafting, EXTRACELLULAR matrix proteins, TISSUE engineering, ENDOTHELIAL cells, BISPECIFIC antibodies, PERICYTES |
| مستخلص: |
Bioengineered tissues or organs produced using matrix proteins or components derived from xenogeneic sources pose risks of allergic responses, immune rejection, or even autoimmunity. Here, we report successful xeno-free isolation, expansion, and cryopreservation of human endothelial cells (EC), fibroblasts (FBs), pericytes (PCs), and keratinocytes (KCs). We further demonstrate the bioprinting of a human skin substitute with a dermal layer containing xeno-free cultured human EC, FBs, and PCs in a xeno-free bioink containing human collagen type I and fibronectin layered in a biocompatible polyglycolic acid mesh and subsequently seeded with xeno-free human KCs to form an epidermal layer. Following implantation of such bilayered skin grafts on the dorsum of immunodeficient mice, KCs form a mature stratified epidermis with rete ridge-like structures. The ECs and PCs form human EC-lined perfused microvessels within 2 weeks after implantation, preventing graft necrosis, and eliciting further perfusion of the graft by angiogenic host microvessels. As proof-ofconcept, we generated 12 individual grafts using a single donor of all four cell types. In summary, we describe the fabrication of a bioprinted vascularized bilayered skin substitute under completely xeno-free culture conditions demonstrating feasibility of a xeno-free approach to complex tissue engineering. [ABSTRACT FROM AUTHOR] |
|
Copyright of Bioengineering & Translational Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder