التفاصيل البيبلوغرافية
العنوان: |
3D bioprinting of an implantable xeno-free vascularized human skin graft. |
المؤلفون: |
Baltazar, Tania, Bo Jiang, Moncayo, Alejandra, Merola, Jonathan, Albanna, Mohammad Z., Saltzman, W. Mark, Pober, Jordan S. |
المصدر: |
Bioengineering & Translational Medicine; Jan2023, Vol. 8 Issue 1, p1-16, 16p |
مصطلحات موضوعية: |
BIOPRINTING, SKIN grafting, EXTRACELLULAR matrix proteins, TISSUE engineering, ENDOTHELIAL cells, BISPECIFIC antibodies, PERICYTES |
مستخلص: |
Bioengineered tissues or organs produced using matrix proteins or components derived from xenogeneic sources pose risks of allergic responses, immune rejection, or even autoimmunity. Here, we report successful xeno-free isolation, expansion, and cryopreservation of human endothelial cells (EC), fibroblasts (FBs), pericytes (PCs), and keratinocytes (KCs). We further demonstrate the bioprinting of a human skin substitute with a dermal layer containing xeno-free cultured human EC, FBs, and PCs in a xeno-free bioink containing human collagen type I and fibronectin layered in a biocompatible polyglycolic acid mesh and subsequently seeded with xeno-free human KCs to form an epidermal layer. Following implantation of such bilayered skin grafts on the dorsum of immunodeficient mice, KCs form a mature stratified epidermis with rete ridge-like structures. The ECs and PCs form human EC-lined perfused microvessels within 2 weeks after implantation, preventing graft necrosis, and eliciting further perfusion of the graft by angiogenic host microvessels. As proof-ofconcept, we generated 12 individual grafts using a single donor of all four cell types. In summary, we describe the fabrication of a bioprinted vascularized bilayered skin substitute under completely xeno-free culture conditions demonstrating feasibility of a xeno-free approach to complex tissue engineering. [ABSTRACT FROM AUTHOR] |
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قاعدة البيانات: |
Complementary Index |