التفاصيل البيبلوغرافية
| العنوان: |
Green Tea Catechol (−)-Epigallocatechin Gallate (EGCG) Conjugated with Phenylalanine Shows Enhanced Autophagy Stimulating Activity in Human Aortic Endothelial Cells. |
| المؤلفون: |
Lee, Taegum, Oh, Yeonji, Kim, Mi Kyoung, Chong, Youhoon |
| المصدر: |
Planta Medica; Apr2023, Vol. 89 Issue 4, p423-432, 10p |
| مصطلحات موضوعية: |
ENDOTHELIAL cells, FLAVONOIDS, PHENYLALANINE, COMBINATION drug therapy, POLYPHENOLS, STAINS & staining (Microscopy), AUTOPHAGY, APOPTOSIS, GREEN tea, CELLULAR aging, OXIDATIVE stress, AORTA, MOLECULAR structure |
| مستخلص: |
(−)-Epigallocatechin gallate (EGCG) is one of the autophagy stimulators that have been reported to protect vascular endothelial cells from oxidative stress-induced damage. In this study, we attempted potentiation of the autophagy-stimulating activity of EGCG in human aortic epithelial cells (HAECs) by using the EGCG-phenylalanine conjugate, E10. Autophagy-stimulating activity of E10 was evaluated by LC3-II measurement in the absence and presence of the lysosomal blocker chloroquine, CTYO-ID staining, and reporter assay using tandem fluorescence-tagged LC3. These experiments revealed significantly enhanced autophagic flux stimulation in HAECs by E10 compared with EGCG. Further elaboration of E10 showed that activation of AMPK through phosphorylation as the major mechanism of its autophagy stimulation. Like other autophagy stimulators, E10 protected HAECs from lipotoxicity as well as accompanying endothelial senescence. Finally, stimulation of autophagy by E10 was shown to protect HAECs from oxidative stress-induced apoptosis. These findings collectively suggest potential clinical implications of E10 for various cardiovascular complications through stimulation of autophagy. [ABSTRACT FROM AUTHOR] |
|
Copyright of Planta Medica is the property of Georg Thieme Verlag Stuttgart and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
