التفاصيل البيبلوغرافية
| العنوان: |
抑制 lncR-HOXA-AS3 表达的人前列腺癌细胞增殖、 迁移、侵袭能力及 EMT 相关蛋白表达变化 . (Chinese) |
| Alternate Title: |
Proliferation, migration, invasion abilities and expression changes of EMT-associated proteins of human prostate cancer cell line LNCaP with inhibition of lncR-HOXA-AS3 expression. (English) |
| المؤلفون: |
朱研峰, 刘志飞, 邢力永, 孟建利, 谢华, 邓刚, 雷竹卿 |
| المصدر: |
Shandong Medical Journal; 2/25/2023, Vol. 63 Issue 6, p6-10, 5p |
| Abstract (English): |
Objective To observe the proliferation, migration, invasion abilities and expression changes of epithelial-mesenchymal transformation (EMT) -related proteins in human prostate cancer (PCa) cell line LNCaP with inhibition of expression of long non-coding RNA (lncR) HOXA-AS3. Methods The cells in this study were human PCa cell line LNCaP. LNCaP cells in the logarithmic growth phase were divided into two groups： observation group (transfected with lncRHOXA-AS3 silencing plasmid si-HOXA-AS3) and control group (transfected with negative control plasmid si-NC) . At 48 h of transfection, lncR-HOXA-AS3 was detected by qRT-PCR. At 24, 48 and 72 h of transfection, the cell proliferation activity (optical density OD value) was measured by CCK-8 assay. At 48 h of transfection, the cell migration ability was observed by cell Scratch test. At 48 h of transfection, the cell invasion ability was observed by Transwell test. Western blotting was used to detect the EMT-associated proteins E-cadherin, Vimentin, and Fibronectin in the two groups at 48 h after transfection. Results Compared with RWPE-1, the relative expression levels of lncR-HOXA-AS3 in DU-145, LNCaP and C4-2B increased (all P<0. 05). Compared with the control group, the relative expression of lncR-HOXA-AS3 in the observation group decreased at 48 h after transfection (P<0. 05) . Compared with the control group, the OD values of the observation group decreased at 24, 48 and 72 h after transfection (all P<0. 05). Compared with the control group, the scar healing rate and the number of invasive transmembrane cells in the observation group were lower at 48 h after transfection (all P<0. 05). Compared with the control group, the relative expression of E-cadherin protein increased and the relative expression levels of Vimentin and Fibronectin proteins decreased at 48 h after transfection in LNCaP cells of the observation group (all P<0. 05). Conclusion Silencing the expression of lncR-HOXA-AS3 can inhibit the proliferation, migration and invasion of LNCaP cells. The mechanism may be that lncR-HOXA-AS3 promotes the expression of E-cadherin protein in LNCaP cells and inhibits the expression levels of Vimentin and Fibronectin proteins. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的 观察抑制长链非编码RNA (long non-coding RNA，lncR) HOXA-AS3表达的人前列腺癌 (prostate cancer， PCa) 细胞系 LNCaP 增殖、迁移、侵袭能力及上皮间质转化 (epithelial-mesenchymal transition， EMT) 相关蛋白的 表达变化。方法 本研究受试细胞为人 PCa 细胞系 LNCaP。将对数生长期 LNCaP 细胞分为 2 组，分别转染 lncRHOXA-AS3 沉默质粒 si-HOXA-AS3 (观察组) 和阴性对照质粒 si-NC (对照组) ，转染 48 h 时采用 qRT-PCR 法检测 ln⁃ cR-HOXA-AS3，分别于转染24、48、72 h时采用CCK8实验测算细胞增殖活性 (光密度OD值) ，转染48 h时采用细胞 划痕实验观察细胞迁移能力，转染 48 h时采用 Transwell 实验观察细胞侵袭能力，转染 48 h时采用 WESTERN Blot⁃ ting 法检测两组 EMT 相关蛋白 E-钙黏蛋白 (E-cadherin) 、波形蛋白 (Vimentin) 、纤维连接蛋白 (Fibronectin) 。结果 与 RWPE-1 相比，DU-145、LNCaP、C4-2B 中 lncR-HOXA-AS3 相对表达量均升高 (P 均<0. 05) 。与对照组相比，转染 48 h时观察组细胞 lncR-HOXA-AS3相对表达量降低 (P<0. 05) 。与对照组比较，转染 24、48、72 h时观察组细胞 OD 值降低 (P均<0. 05) 。与对照组比较，转染48 h时观察组划痕愈合率小、侵袭穿膜细胞数目少 (P均<0. 05) 。与对照 组比较，转染48 h时观察组LNCaP细胞E-cadherin蛋白相对表达量升高，Vimentin、Fibronectin蛋白相对表达量表达 降低 (P 均<0. 05) 。结论 沉默 lncR-HOXA-AS3 表达能抑制 LNCaP 细胞的增殖、迁移及侵袭，其机制可能为 lncRHOXA-AS3促进LNCaP细胞E-cadherin蛋白表达、抑制Vimentin及Fibronectin蛋白表达。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
