التفاصيل البيبلوغرافية
| العنوان: |
Multi-angle insitu dynamic light scattering at a neutron spin echo spectrometer. |
| المؤلفون: |
Vögl, F., Balacescu, L., Holderer, O., Pasini, S., Staringer, S., Brandl, G., Ossovyi, V., Feilbach, H., Müller-Buschbaum, P., Stadler, A. M., Fitter, J., Schrader, T. E. |
| المصدر: |
Review of Scientific Instruments; Mar2023, Vol. 34 Issue 3, p1-10, 10p |
| مصطلحات موضوعية: |
NEUTRON scattering, NEUTRON measurement, FREE-space optical technology, SPECTROMETERS, MOMENTUM transfer, LIGHT scattering, NEUTRON reflectometry, STOVES |
| مستخلص: |
A new sample environment, called Bio-Oven, has been built for the Neutron Spin Echo (NSE) Spectrometer J-NSE Phoenix. It provides active temperature control and the possibility to perform Dynamic Light Scattering (DLS) measurements during the neutron measurement. DLS provides diffusion coefficients of the dissolved nanoparticles, and thus one can monitor the aggregation state of the sample on a time scale of minutes during the spin echo measurement times on the order of days. This approach helps to validate the NSE data or to replace the sample when its aggregation state influences the spin echo measurement results. The new Bio-Oven is an in situ DLS setup based on optical fibers decoupling the free space optics around the sample cuvette in a lightproof casing from the laser sources and the detectors. It collects light from three scattering angles simultaneously. Six different values of momentum transfer can be accessed by switching between two different laser colors. Test experiments were performed with silica nanoparticles with diameters ranging from 20 nm up to 300 nm. Their hydrodynamic radii were determined from DLS measurements and compared with the ones obtained by a commercial particle sizer. It was demonstrated that also the static light scattering signal can be processed and gives meaningful results. The protein sample apomyoglobin was used for a long-term test and in a first neutron measurement using the new Bio-Oven. The results prove that the aggregation state of the sample can be followed using in situ DLS along with the neutron measurement. [ABSTRACT FROM AUTHOR] |
|
Copyright of Review of Scientific Instruments is the property of American Institute of Physics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Find this article in full text from Scitation.