التفاصيل البيبلوغرافية
| العنوان: |
Effect of Methionine on Gene Expression in Komagataella phaffii Cells. |
| المؤلفون: |
Ianshina, Tatiana, Sidorin, Anton, Petrova, Kristina, Shubert, Maria, Makeeva, Anastasiya, Sambuk, Elena, Govdi, Anastasiya, Rumyantsev, Andrey, Padkina, Marina |
| المصدر: |
Microorganisms; Apr2023, Vol. 11 Issue 4, p877, 15p |
| مصطلحات موضوعية: |
GENE expression, METHIONINE, PROTEIN biotechnology, RECOMBINANT proteins, AMINO acids, AMINO acid metabolism |
| مستخلص: |
Komagataella phaffii yeast plays a prominent role in modern biotechnology as a recombinant protein producer. For efficient use of this yeast, it is essential to study the effects of different media components on its growth and gene expression. We investigated the effect of methionine on gene expression in K. phaffii cells using RNA-seq analysis. Several gene groups exhibited altered expression when K. phaffii cells were cultured in a medium with methanol and methionine, compared to a medium without this amino acid. Methionine primarily affects the expression of genes involved in its biosynthesis, fatty acid metabolism, and methanol utilization. The AOX1 gene promoter, which is widely used for heterologous expression in K. phaffii, is downregulated in methionine-containing media. Despite great progress in the development of K. phaffii strain engineering techniques, a sensitive adjustment of cultivation conditions is required to achieve a high yield of the target product. The revealed effect of methionine on K. phaffii gene expression is important for optimizing media recipes and cultivation strategies aimed at maximizing the efficiency of recombinant product synthesis. [ABSTRACT FROM AUTHOR] |
|
Copyright of Microorganisms is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder