التفاصيل البيبلوغرافية
| العنوان: |
Gelatin Micro/Nanoparticles-Based Delivery of Urease and Omp31 in Mice Has a Protective Role Against Brucella melitensis 16 M Infection. |
| المؤلفون: |
Sattarahmady, N., Alamian, S., Abkar, M. |
| المصدر: |
BioNanoScience; Jun2023, Vol. 13 Issue 2, p686-694, 9p |
| مستخلص: |
Brucellosis is one of the most endemic diseases in many regions of the world. Due to its serious medical and economic consequences, many attempts have been made to prevent infection in domestic animals through vaccines. In this study, vaccine formulations of urease-loaded gelatin (urease/gelatin) micro/nanoparticles (MNPs) and Omp31-loaded gelatin (Omp31/gelatin) MNPs against brucellosis were developed. Urease/gelatin MNPs and Omp31/gelatin MNPs, separately or in combination (MNPs cocktail), were orally administered. The formulations comprised 165-nm to 1.3-µm particles. Antibody detection, cytokine measurement, and lymphocyte proliferation assay were performed. Finally, the immunized mice were challenged with the virulent B. melitensis 16 M. All the immunized mice elicited titers of specific IgG and IgA. According to cytokine assay and antibody isotypes, oral administration with all vaccine formulations stimulated a Th1-Th17 immune response. In lymphocyte proliferation assay, splenocytes from all-immunized mice indicated a robust recall proliferative response. The MNPs cocktail conferred protection against B. melitensis challenge equivalent to that of vaccine strain B. melitensis Rev.1. In comparison to gelatin/Omp31 MNPs alone, MNPs cocktail induced only a low increase in protection level. Altogether, the results showed that MNPs cocktail might be a promising candidate for the subunit vaccines development against brucellosis. Furthermore, gelatin MNPs are a suitable delivery system for orally-administered Brucella antigens. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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