التفاصيل البيبلوغرافية
| العنوان: |
Circulating extracellular particles from severe COVID-19 patients show altered profiling and innate lymphoid cell-modulating ability . |
| المؤلفون: |
Forte, Dorian, Pellegrino, Roberto Maria, Trabanelli, Sara, Tonetti, Tommaso, Ricci, Francesca, Cenerenti, Mara, Comai, Giorgia, Tazzari, Pierluigi, Lazzarotto, Tiziana, Buratta, Sandra, Urbanelli, Lorena, Narimanfar, Ghazal, Alabed, Husam B. R., Mecucci, Cristina, Manna, Gaetano La, Emiliani, Carla, Jandus, Camilla, Ranieri, Vito Marco, Cavo, Michele, Catani, Lucia |
| المصدر: |
Frontiers in Immunology; 2023, p1-16, 16p |
| مصطلحات موضوعية: |
COVID-19, INNATE lymphoid cells, GEL permeation chromatography, EXTRACELLULAR vesicles, PARTIAL pressure |
| مستخلص: |
Introduction: Extracellular vesicles (EVs) and particles (EPs) represent reliable biomarkers for disease detection. Their role in the inflammatory microenvironment of severe COVID-19 patients is not well determined. Here, we characterized the immunophenotype, the lipidomic cargo and the functional activity of circulating EPs from severe COVID-19 patients (Co-19-EPs) and healthy controls (HC-EPs) correlating the data with the clinical parameters including the partial pressure of oxygen to fraction of inspired oxygen ratio (PaO2/FiO2) and the sequential organ failure assessment (SOFA) score. Methods: Peripheral blood (PB) was collected from COVID-19 patients (n=10) and HC (n=10). EPs were purified from platelet-poor plasma by size exclusion chromatography (SEC) and ultrafiltration. Plasma cytokines and EPs were characterized by multiplex bead-based assay. Quantitative lipidomic profiling of EPs was performed by liquid chromatography/mass spectrometry combined with quadrupole time-of-flight (LC/MS Q-TOF). Innate lymphoid cells (ILC) were characterized by flow cytometry after co-cultures with HC-EPs or Co-19-EPs. Results: We observed that EPs from severe COVID-19 patients: 1) display an altered surface signature as assessed by multiplex protein analysis; 2) are characterized by distinct lipidomic profiling; 3) show correlations between lipidomic profiling and disease aggressiveness scores; 4) fail to dampen type 2 innate lymphoid cells (ILC2) cytokine secretion. As a consequence, ILC2 from severe COVID-19 patients show a more activated phenotype due to the presence of Co-19-EPs. Discussion: In summary, these data highlight that abnormal circulating EPs promote ILC2-driven inflammatory signals in severe COVID-19 patients and support further exploration to unravel the role of EPs (and EVs) in COVID-19 pathogenesis. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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