التفاصيل البيبلوغرافية
| العنوان: |
In vitro production of buffalo embryos in Colombia – results of the addition of growth factors and antioxidants to defined culture medium. |
| Alternate Title: |
In vitro proizvodnja zametaka bizona u Kolumbiji - rezultati dodavanja faktora rasta i antioksidansa definiranom mediju za uzgoj zametaka. (Bosnian) |
| المؤلفون: |
Chavez-Rodriguez, A., Dubeibe-Marin, D. F., Crudeli, G., Berdugo-Gutiérrez, J. A. |
| المصدر: |
Veterinarska Stanica; 2023, Vol. 54 Issue 3, p293-299, 7p |
| مصطلحات موضوعية: |
WATER buffalo, CATTLE embryos, ANTIOXIDANTS, CULTURE media (Biology), GROWTH factors |
| مصطلحات جغرافية: |
COLOMBIA |
| Abstract (English): |
To increase the success rate of the in vitro production (IVP) system of embryos in buffalo, it is necessary to improve parameters. This field requires more research since its results are low compared to cattle. In this study carried out in Colombia, a modification of the methodology to produce buffalo IVP embryos is described, and 24 non-lactating females, aged 4 to 11 years old were included. Follicular wave was synchronised by ablation of the follicles within the ovary, seven days later all follicles were aspirated to obtain oocytes for maturation, fertilisation and embryo production. For all culture media used, animal protein was avoided. Culture media were supplemented with growth factors and other molecules associated with embryonic development. Special care was taken in adding supplements to protect the zygotes against the harmful effect of oxygen free radicals in the culture medium. Two aspiration series were performed 15 days apart. The average number of oocytes obtained by aspiration was 11.33, the number of blastocysts was 3.05, and the obtained blastocyst production rate (36.2%) was higher than literature reports for this species; we hypothesise that this increase is associated with the supplements added. It was possible to observe a female that produced 50 oocytes. Subsequent research is needed to evaluate the effect of the medium on embryo quality and pregnancy rates. A cost analysis of the new proposed medium is required, since all supplements must be imported and handled with a cold chain of up to -80°C, making it more expensive and restricting widespread use. Finally, it is shown that with three embryos per aspiration, at least one pregnancy can be successful after the aspiration, which is one of the objectives of the IVP system. [ABSTRACT FROM AUTHOR] |
| Abstract (Bosnian): |
Za povećanje djelotvornosti sustava in vitro proizvodnje (IVP) zametaka u bizona, potrebno je poboljšati parametre. Budući da su rezultati slabi u usporedbi s govedima ovo područje zahtijeva više istraživanja. U ovom radu, provedenom u Kolumbiji, modifikacija metodologije za proizvodnju IVP zametaka bizona opisana je na sljedeći način: korištene su 24 ženke u dobi između 4 i 11 godina, a koje nisu bile u laktaciji. Folikularni val je sinkroniziran ablacijom folikula unutar jajnika, a nakon sedam dana obavljena je aspiracija svih folikula ženki za dobivanje oocista za dozrijevanje, oplodnju i proizvodnju embrija. Za sve rabljene podloge izbjegavan je životinjski protein. Podloge su obogaćene faktorima rasta i drugim molekulama povezanima s razvojem embrija. Posebna pozornost posvećena je pri dodavanju dodataka za zaštitu zigota od štetnih učinaka slobodnih radikala u podlozi. Obavljene su dvije aspiracijske serije u razmaku od 15 dana. Prosječni je broj oocista dobivenih aspiracijom bio 11,33, broj blastocista bio je 3,05, a dobivena je stopa proizvodnje blastocista (36,2 %) bolja je u usporedbi s onom koju su objavili drugi autori u državi, za istu vrstu. Pretpostavljamo da je ovo povećanje povezano s dodanim dodatcima, bilo je moguće promatrati ženku koja je proizvela 50 oocista. Naknadno je istraživanje potrebno za procjenu učinka podloge na kvalitetu zametaka i postotka gravidnosti; potrebna je i analiza troškova novog predloženog medija, jer se svi dodatci moraju uvesti u Kolumbiju i čuvati u hladnim uvjetima do minus 80 oC, što ih čini skupljima, a ograničava i njihovu široku primjenu. Na kraju našeg istraživanja uočeno je da s tri zametka po aspiraciji u ove vrste nije teško postići najmanje jednu gravidnost, a to je i jedan od ciljeva IVP-a. [ABSTRACT FROM AUTHOR] |
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