التفاصيل البيبلوغرافية
العنوان: |
Extraction and Refining Process of Polysaccharide from Tricholoma matsutake and Verification of Its Whitening Effect. |
المؤلفون: |
Quan AN, Guoqing LI, Xuetao LI, Ruijun JU, Xue SHAO |
المصدر: |
Medicinal Plant; Feb2023, Vol. 14 Issue 1, p19-24, 6p |
مصطلحات موضوعية: |
LIPOXINS, MELANOGENESIS, MELANINS, SURVIVAL rate, PHENOL oxidase, POLYSACCHARIDES |
مستخلص: |
Objectives] The paper was to determine the extraction and refining process of polysaccharide from Tricholoma matsutake, and to verify its whitening effect. [Methods] With T. matsutake as the research object, the effects of eluent concentration, loading amount and diameter-to-height ratio on the refining process of T. matsutake polysaccharide were explored by orthogonal test, to optimize the dynamic elution conditions of T. matsutake polysaccharide. The survival rate of mouse melanoma cells (B16), inhibition of melanin synthesis and inhibition of tyrosinase activity of crude extract Al and refined extracts A2-A4 were tested to verify the whitening effect of T. matsutake. [Results] The best extraction and refining process for T. matsutake polysaccharide was: eluent 40% ethanol (volume fraction), loading amount 30 mg/g (polysaccharide-resin), diameter-to-height ratio 1:8. Under this condition, the extraction rate of T. matsutake can reach up to 33% (A3). The whitening efficacy test showed that crude extract Al and refined extracts A2-A4 had good whitening effects, and the whitening effects were A33:A4 >A1 > A2. [Conclusions] The study provides a reference for the application of T. matsutake polysaccharide in cosmetics. [ABSTRACT FROM AUTHOR] |
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قاعدة البيانات: |
Complementary Index |