التفاصيل البيبلوغرافية
| العنوان: |
大豆皂甙对杂交黄颡鱼生长、免疫及肠道健康的影响. (Chinese) |
| Alternate Title: |
SOYA SAPONINS ON GROWTH, IMMUNITY AND INTESTINAL HEALTH OF YELLOW CATFISH HYBRID (PELTEOBAGRUS FULVIDRACO♀×PELTEOBAGRUS VACHELLI♂). (English) |
| المؤلفون: |
王岳松, 李 鸿, 矣林圆, 刘婧文, 沈志刚, 杨慧君, 袁勇超 |
| المصدر: |
Journal of Hydrobiology; Sep2023, Vol. 47 Issue 9, p1386-1395, 10p |
| Abstract (English): |
In order to investigate the effects of soyas aponins on growth, immunity and intestinal health of hybrid yellow catfish (Pelteobagrus fulvidraco♀×Pelteobagrus vachelli♂), six isonitroid and isolipid diets supplemented with 0, 0.20%, 0.40%, 0.80%, 1.60% and 4.00% soy saponin with fish meal, shrimp meal and corn protein concentrate as protein sources were fed to hybrid yellow catfish with an initial weight of (1.0±0.17) g. They were divided into D0, D0.2, D0.4, D0.8, D1.6 and D4.0, with three replicates in each group and 30 fish in each replicate. After 60-day feeding experiment, the changes of growth index, body composition, serum immunity, antioxidant enzyme activity and intestinal inflammatory factor expression levels were measured. The results showed that the weight gain rate and specific growth rate of treated groups were significantly decreased (P<0.05), the content of body composition protein and muscle fat decreased first and then increased, while the content of body fat and muscle protein increased and then decreased. With the increase of saponins level, T-AOC in serum continuously decreased, AKP, NO, CAT activities increased at first and then decreased while ACP activities decreased at first and then increased. We found that the activities of ACP in liver of D0.8, D1.6 and D4.0 groups were significantly higher than other groups and the activities of CAT in liver of D0 and D4.0 groups were significantly lower than other groups (P<0.05). Besides, the activity of liver T-AOC increased first and then decreased, while the content of liver MDA decreased with the increase of saponin level. The content of liver MDA in the treated groups with saponin were significantly lower compared to D0 group. The activity of liver T-SOD showed a tendency to decrease first and then increase. With the increasing of the level of saponins, the mRNA levels of TGF-β and Il-10 in distal intestine were reduced, while that of Il-1β, IL-8, Il-15 and TNF-α were increased first and reduced later. To sum up, the soybean saponins added to the diet had obvious negative effects on the growth performance, immunity, anti-oxidation ability and intestinal health of the hybrid yellow catfish. When the soybean saponins level exceeded 0.40%, the hybrid yellow catfish showed severe oxidative stress, immune system disorder, liver injury, up-regulation of intestinal pro-inflammatory factors and down-regulation of anti-inflammatory factors. Therefore, it is necessary to limit the content of soybean saponins to 0.40% by replacing fish meal with soybean meal. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
为探究大豆皂甙对杂交黄颡鱼(Pelteobagrus fulvidraco♀×Pelteobagrus vachelli♂)生长、免疫及肠道健 康的影响, 在鱼粉、虾粉及玉米浓缩蛋白为蛋白源的基础饲料中分别添加0、0.20%、0.40%、0.80%、 1.60%和4.00%的大豆皂甙, 配制成6种等氮等脂的饲料饲喂初始质量为(1.0±0.17) g的杂交黄颡鱼, 分别记为 D0、D0.2、D0.4、D0.8、D1.6和D4.0等处理组, 每组设3个重复, 每个重复30尾鱼。60d饲养试验结束后测定 生长指标、体组成、血清免疫、抗氧化酶活性和肠道炎症因子表达量等变化。结果显示: (1) 与D0组相比, 添加大豆皂甙的处理组增重率和特定生长率均显著性降低(P<0.05), 饲料系数显著性升高(P<0.05)。试验鱼 体蛋白含量和肌肉脂肪含量先降低后升高, 体脂含量和肌肉蛋白含量先上升后下降。(2) 随着饲料皂甙水平 的提高, 血清T-AOC持续降低, AKP、NO和CAT活性先升高后降低, ACP活性先降低后升高。(3) D0.8、 D1.6和D4.0组试验鱼肝脏ACP活性显著高于其他组(P<0.05); D0和D4.0组试验鱼肝脏CAT活性显著低于其他 组(P<0.05); 肝脏T-AOC活性表现出先升高后降低的趋势; 肝脏MDA含量随皂甙水平升高逐渐降低, 添加皂 甙的处理组均显著低于D0组(P<0.05); 肝脏T-SOD表现出先降低后升高的趋势。(4) 随着日粮皂甙水平的不 断提高, 远端肠道TGF-β和IL-10 mRNA表达不断下调, IL-1β、IL-8、IL-15和TNF-α mRNA表达先上调后下 调。综上所述, 在饲料中添加一定水平的大豆皂甙对杂交黄颡鱼的生长性能、免疫及抗氧化能力和肠道健康 产生了明显的负面影响, 当大豆皂甙水平超过0.40%后, 杂交黄颡鱼生长和免疫均受到抑制, 出现了典型的肠 炎现象。因此在黄颡鱼商业养殖中选择饲料需要控制皂甙含量不超过0.40%。. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
