التفاصيل البيبلوغرافية
| العنوان: |
白藜芦醇可调控运动性疲劳大鼠的糖异生. (Chinese) |
| Alternate Title: |
Effect of resveratrol on gluconeogenesis in exercise-induced fatigue rats. (English) |
| المؤلفون: |
阮 蓉, 娄旭佳, 金其贯, 章立冰, 徐 尚, 胡玉龙 |
| المصدر: |
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu; 3/18/2024, Vol. 28 Issue 8, p1229-1234, 6p |
| مصطلحات موضوعية: |
CREB protein, SWIMMING training, FATIGUE (Physiology), ENZYME-linked immunosorbent assay, CREATINE kinase, PYRUVATES, MONOCARBOXYLATE transporters, LACTATES, PEROXISOME proliferator-activated receptors |
| Abstract (English): |
BACKGROUND: Resveratrol is a natural antioxidant extracted from plants. Its mechanism of improving exercise-induced fatigue mainly focuses on the protective effect against oxidative stress and inflammation. In this study, the protective mechanism of resveratrol on exercise-induced fatigue was mainly discussed from the perspective of gluconeogenesis. OBJECTIVE: To investigate the effect of resveratrol on gluconeogenesis in exercise-induced fatigue rats. METHODS: After 1 week of adaptive training, male Sprague-Dawley rats were randomly divided into 4 groups with 12 rats in each group: blank control group, resveratrol group, exercise group, resveratrol + exercise group. Weight-bearing swimming training was used to simulate long-term medium-high intensity exercise. After swimming with a weight of 5% for 1 hour every day, 50 mg/kg resveratrol solution or the same volume of dimethyl sulfoxide solvent were given orally, 6 days a week, for a total of 6 weeks. Samples were collected 24 hours after the last exercise, and the levels of urea nitrogen, creatine kinase, blood glucose, liver glycogen and lactic acid and pyruvate in liver tissue were detected by the kit. The activity of phosphoenolpyruvate carboxykinase was detected by microassay, and the activity of glucose-6-phosphatase was detected by enzyme-linked immunosorbent assay. Real-time fluorescence quantitative PCR was used to detect the gene expression of silent information regulator 1, cAMP-response element binding protein and peroxisome proliferator-activated receptor-γ coactivator-1α. RESULTS AND CONCLUSION: In the exercise group, plasma urea nitrogen and creatine kinase levels of rats were significantly increased (both P < 0.05), liver lactate and pyruvate levels and lactate/pyruvate ratio were significantly increased (all P < 0.01), and blood glucose and liver glycogen contents were significantly decreased (both P < 0.01). Resveratrol supplementation could effectively improve the above conditions. Exercise significantly decreased the activities of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase (P < 0.01, P < 0.05), and resveratrol supplementation significantly increased the activity of phosphoenolpyruvate carboxykinase in liver tissue (P < 0.01). The mRNA expression levels of silent information regulator 1, cAMP-response element binding protein and peroxisome proliferator-activated receptor-γ coactivator-1α in liver tissue of the exercise group were significantly decreased (all P < 0.01), while resveratrol supplementation could significantly increase the gene expression levels of this pathway. To conclude, resveratrol can relieve exercise-induced fatigue caused by long-term medium-high intensity exercise, and its mechanism may be related to up-regulating the gluconeogenesis regulatory pathway, improving rate-limiting enzyme activity, promoting liver gluconeogenesis, and increasing blood glucose and liver glycogen levels. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
背景：白藜芦醇是从植物中提取而来的天然抗氧化剂, 其改善运动性疲劳的机制主要集中在氧化应激和炎症反应的保护作用上, 此次研究 主要从糖异生角度探讨其对运动性疲劳的保护机制。 目的：探讨白藜芦醇对运动性疲劳大鼠糖异生的影响。 方法：SD雄性大鼠适应性训练1周后随机分为4组, 即空白对照组、白藜芦醇组、运动组、白藜芦醇+运动组, 每组12只。空白对照组和白 藜芦醇组正常饲养, 不进行游泳训练；白藜芦醇+运动组和运动组采用负重游泳训练模拟长时间中高强度运动, 每天负重5%游泳1 h后, 分 别灌胃50 mg/kg的白藜芦醇溶液或等体积的二甲基亚砜溶剂, 每周6 d, 共6周。末次运动后24 h取材, 试剂盒检测尿素氮、肌酸激酶、血 糖、肝糖原以及肝脏组织中乳酸、丙酮酸水平；微量法检测磷酸烯醇式丙酮酸羧激酶活性, 酶联免疫吸附法检测葡萄糖-6-磷酸酶活性； RT-PCR检测SIRT1、CREB和PGC-1α的基因表达水平。 结果与结论：①运动组大鼠血浆尿素氮和肌酸激酶水平明显升高(均P < 0.05), 肝脏乳酸、丙酮酸水平和乳酸/丙酮酸比值明显升高(均P < 0.01), 血糖和肝糖原水平明显降低(均P < 0.01)；补充白藜芦醇可有效改善以上情况；②运动使大鼠肝脏组织糖异生关键酶磷酸烯醇式丙酮 酸羧激酶和葡萄糖-6-磷酸酶的活性明显降低(P < 0.01, P < 0.05), 补充白藜芦醇可明显提高磷酸烯醇式丙酮酸羧激酶活性(P < 0.01)；③运 动组肝脏组织SIRT1、CREB和PGC-1α的mRNA表达水平明显降低(均P < 0.01), 补充白藜芦醇可使该通路基因表达水平明显升高；④提示白藜 芦醇能缓解长时间中高强度运动导致的运动性疲劳, 其机制可能与上调糖异生调控通路、提高限速酶活性、促进肝脏糖异生、升高血糖和 肝糖原水平有关。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
