التفاصيل البيبلوغرافية
العنوان: |
Radiocomplexation, Biological Evaluation, and Characterization of [99mTc]-5-[(3-Carboxy-4-hydroxyphenyl)diazenyl]-2-hydroxybenzoic Acid as a Novel Agent for Imaging of Ulcerative Colitis in Mice. |
المؤلفون: |
Sanad, M. H., Eyssa, H. M., Marzook, F. A., Farag, A. B., Elrefaei, A., Fouzy, A. S. M., Challan, S. B. |
المصدر: |
Radiochemistry; Jun2023, Vol. 65 Issue 3, p378-386, 9p |
مصطلحات موضوعية: |
ULCERATIVE colitis, THIN layer chromatography, HIGH performance liquid chromatography, RADIOCHEMICAL purification, MICE |
مستخلص: |
A procedure was developed for preparing [99mTc]-tricarbonyl-5-[(3-carboxy-4-hydroxyphenyl)diazenyl]-2-hydroxybenzoic acid complex ([99mTc]-tricarbonyl-Olsalazine), for ulcerative colitis (UC) imaging. High yield and 98.5% radiochemical purity of [99mTc]-tricarbonyl-Olsalazine were reached under optimized conditions. Thin layer chromatography (TLC) was used for the purification of the labeled compound. Reversed-phase high-performance liquid chromatography (RP-HPLC) was also used. A biodistribution study performed with a microbial mouse model of UC showed that the colon localization of [99mTc]-tricarbonyl-Olsalazine was the highest (77 ± 1.7% ID/g) at 120 min post injection. The agent was still present in the ulcerated colon for up to 24 h post injection (75% ID/g). Such level was not reached in previous studies. Thus, [99mTc]-tricarbonyl-Olsalazine can be used as a radiotracer for UC imaging in mice. [ABSTRACT FROM AUTHOR] |
|
Copyright of Radiochemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
قاعدة البيانات: |
Complementary Index |