التفاصيل البيبلوغرافية
| العنوان: |
Genetic Transformation of Tobacco with the Trehalose Synthase Gene fromGrifola frondosaFr. Enhances the Resistance to Drought and Salt in Tobacco. |
| المؤلفون: |
Shu-Zhen Zhang, Ben-Peng Yang, Cui-Lian Feng, Huo-Long Tang |
| المصدر: |
Journal of Integrative Plant Biology; May2005, Vol. 47 Issue 5, p579-587, 9p |
| مصطلحات موضوعية: |
PLANT genetic engineering, GENETIC transformation, EFFECT of stress on plants, TRANSGENIC plants, PLANT morphology, BIOTECHNOLOGY, BOTANY |
| مستخلص: |
Trehalose is a non-reducing disaccharide of glucose that functions as a protectant in the stabilization of biological structures and enhances the tolerance of organisms to abiotic stress. In the present study, we report on the expression of theGrifola frondosaFr. trehalose synthase (TSase)gene for manipulating abiotic stress tolerance in tobacco (Nicotiana tabaccumL.). The expression of the transgene was under the control of two tandem copies of the CaMV3 5 S promoter and was transferred into tobacco byAgrobacterium tumefaciensEHA105. Compared with non-transgenic plants, transgenic plants were able to accumulate high levels of products of trehalose, which were increased up to 2.126–2.556 mg/g FW, although levels were undetectable in non-transgenic plants. This level of trehalose in transgenic plants was 400-fold higher than that of transgenic tobacco plants cotransformed withEscherichia coli TPSandTPPon independent expression cassettes, twofold higher than that of transgenic rice plants transformed with a bi functional fusion gene (TPSP)of the trehalose-6-phosphate (T-6-P) synthase (TPS) and T-6-P phosphatase (TPP) ofE. coli, and 12-fold higher than that of transgenic tobacco plants transformed the yeastTPS1gene. It has been reported that transgenic plants withE. coli TPSand/orTPPwere severely stunted and had morphological alterations of their roots. Interestingly, our transgenic plants have obvious morphological changes, including thick and deep-coloured leaves, but show no growth inhibition; moreover, these morphological changes can restore to normal type in T2 progenies. Trehalose accumulation in35S–35S:TSaseplants resulted in increased tolerance to drought and salt, as shown by the results of tests on drought, salt tolerance, and drought physiological indices, such as water content in excised leaves, malondialdehyde content, chlorophyllaandbcontents, and the activity of superoxide dismutase and peroxidase in excised leaves. These results suggest that transgenic plants transformed with theTSasegene can accumulate high levels of trehalose and have enhanced tolerance to drought and salt.(Managing editor: Li-Hui ZHAO) [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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