التفاصيل البيبلوغرافية
| العنوان: |
一株鱼源致病性嗜水气单胞菌 XDMG 的全基因组测序 及比较基因组分析. (Chinese) |
| Alternate Title: |
Whole Genome Sequencing and Comparative Genome Analysis of a Fish-derived Pathogenic Aeromonas Hydrophila Strain XDMG. (English) |
| المؤلفون: |
郭少华, 毛会丽, 刘征权, 付美媛, 赵平原, 马文博, 李旭东, 关建义 |
| المصدر: |
Biotechnology Bulletin; 2023, Vol. 39 Issue 8, p291-306, 16p |
| مصطلحات موضوعية: |
WHOLE genome sequencing, GENETIC engineering, TANDEM repeats, AEROMONAS hydrophila, DNA vaccines, GENETIC software, TRANSFER RNA, SHORT tandem repeat analysis |
| Abstract (English): |
The objectives of this study are to explore the mechanism of pathogenic Aeromonas hydrophila XDMG strain infecting fish, and to compare its genome structure, function and evolutionary relationship with the reference strain. The whole genome of A. hydrophila XDMG strain was sequenced, and the original data obtained by sequencing were assembled, spliced and annotated using Newbler, SeqMan, Glimmer and other software. Based on the constructing phylogenetic tree analysis from housekeeping genes and determining evolutionary relationships, the genomes of A. hydrophila were compared with those of four A. hydrophila strains in different regions. The complete genome sequence of A. hydrophila XDMG strain was 4.99 Mb in length and the GC content was 60.84%. A total of 4 935 coding genes were predicted, 4 137 genes with clear function were found, 99 tRNAs and 3 rRNAs were found; 4 061 genes had ortholog family classification, 3 228 genes were related to gene ontology, and 2 663 genes related to metabolic pathways; 101 tandem repeats and 14 gene islands were predicted in the genome of A. hydrophila XDMG strain, with genes related to three QS systems: AI 1, AI 2, and AI 3. Through genome comparative analysis, A. hydrophila XDMG was closely related to domestic A. hydrophila J-1 strain and US-derived A. hydrophila AL0971 strain; it was more conserved with US-derived A. hydrophila AL0971 in the comparison of virulence genes, outer membrane proteins, and O-antigen gene clusters. Comprehensive genome annotation and comparative analysis of the genome of A. hydrophila XDMG strain provide basic data for the study of functional genomics and genetic engineering vaccines of A. hydrophila. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
探索致病性嗜水气单胞菌 XDMG 侵染鱼类的作用机制，对其基因组结构、功能和进化关系等基本特征与参考菌株 进行比较分析。对嗜水气单胞菌 XDMG 进行全基因组测序，利用 Newbler、SeqMan、Glimmer 等软件对测序得到的原始数据进行组 装、拼接、注释，再基于看家基因构建系统发育树分析，确定进化关系的基础上，与 4 株不同地区的嗜水气单胞菌进行基因组的 比较分析。嗜水气单胞菌 XDMG 全基因组序列长 4.99 Mb，GC 含量为 60.84%，共预测到 4 935 个编码基因，有明确功能的基因有 4 137 个，发现 99 个 tRNA 和 3 个 rRNA ；4 061 个基因具有直系同源族分类，3 228 个基因与基因本体论相关，与代谢通路有关的 基因有 2 663 个 ；在嗜水气单胞菌 XDMG 基因组中预测了 101 个串联重复序列和 14 个基因岛，具有 AI-1、AI-2 和 AI-3 三种 QS 系 统的相关基因。通过基因组对比分析，嗜水气单胞菌 XDMG 与国内嗜水气单胞菌 J-1 菌株和美国来源的嗜水气单胞菌 AL09_71 菌 株具有较近的亲缘关系 ；在毒力基因、外膜蛋白和 O- 抗原基因簇的比较中与美国来源的嗜水气单胞菌 AL09_71 保守性更高。通过 对嗜水气单胞菌 XDMG 基因组进行较为全面的基因组注释及基因组的比较分析，为嗜水气单胞菌功能基因组学、基因工程疫苗方 面的研究提供基础数据。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
