التفاصيل البيبلوغرافية
| العنوان: |
The detection and full genomic characterization of domestic cat Orthohepadnaviruses from Türkiye. |
| المؤلفون: |
Adıgüzel, Ece, Erdem‐Şahinkesen, Eda, Koç, Bahattin Taylan, Demirden, Cansu, Oğuzoğlu, Tuba Çiğdem |
| المصدر: |
Veterinary Medicine & Science; Sep2023, Vol. 9 Issue 5, p1965-1972, 8p |
| مصطلحات موضوعية: |
CATS, GENE amplification, VIRUS diseases, NUCLEIC acids, DATABASES |
| مصطلحات جغرافية: |
TURKEY |
| مستخلص: |
Background: Domestic cat hepadnaviruses (DCHs) have been described as a novel virus that can infect cats. Objective: The aim of our study is the first identification and molecular characterizations of DCH infection in Turkish domestic cats. Methods: The blood, organ and ascites fluid samples from 550 cats were randomly sampled. The presence of DCH nucleic acid was investigated by using both in the literature and newly designed primers. Results: It was found that the hepadnavirus positivity rate is 4% (22/550) in Türkiye. The full genomic characterization was performed on 13 of 22 samples, and others were characterized as nearly full genome. In this study, we highlight that whole blood samples should be also screened for DCH, not only serum samples as has frequently been done in other studies. DCH‐infected cats were also found positive (54.54%, 12/22) for Feline leukaemia virus infection. BLAST results revealed that Turkish DCHs have 86.32%–99.08% homology with strains in the GenBank database, enabling us to construct phylogenetic trees. Conclusions: According to this study's results, it is suggested that this infection should be added to veterinary diagnostic panels worldwide. Additionally, we suggest that our new synthesized primers for the amplification of X gene can also be used for diagnosis. [ABSTRACT FROM AUTHOR] |
|
Copyright of Veterinary Medicine & Science is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder