التفاصيل البيبلوغرافية
| العنوان: |
Development of a cryo-soft x-ray tomography at Taiwan photon source. |
| المؤلفون: |
Lai, Lee-Jene, Lin, Zi-Jing, Wang, Duan-Jen, Yin, Gung-Chian, Hsieh, Chia-Chun, Lin, Yi-Hung, Chen, Bo-Yi, Lee, Chien-Yu |
| المصدر: |
AIP Conference Proceedings; 9/2/2023, Vol. 2990 Issue 1, p1-5, 5p |
| مصطلحات موضوعية: |
SOFT X rays, X-rays, TOMOGRAPHY, PHOTONS, THREE-dimensional imaging, OPTICAL microscopes |
| مستخلص: |
A transmission full-field microscope of cryo-soft X-ray tomography (SXT) was developed as a platform to image three-dimensional frozen hydrated cells in beamline TPS 24A at Taiwan Photon Source (TPS). This microscope consists of two main compositions in a vacuum chamber: The first composition is the optical system of the microscope, and the second composition is the cryogenic cooling system. The optical system includes a high demagnification capillary condenser and an objective of Fresnel zone plate. The cryogenic cooling system consists of sample transferring, robot and rotational sample systems. In our preliminary results, a spatial resolution of around 25∼30 nm half-pitch was obtained from a 2D image of standard calibration Nested L sample, and a clear crista of inner membrane in mitochondria from a frozen lung carcinoma cell was imaged at water window energy of 520 eV. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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