التفاصيل البيبلوغرافية
| العنوان: |
Systems analysis of transcriptome and proteome in retinoic acid/arsenic trioxide-induced cell differentiation/apoptosis of promyelocytic leukemia. |
| المؤلفون: |
Pei-zheng Zheng, Kan-kan Wang, Qun-ye Zhang, Qiu-hua Huang, Yan-zhi Du, Qing-hua Zhangi, Da-kai Xiao, Shu-hong Shen, Sandrine Lmbeaud, Eric Eveno, Chun-jun Zhao, Vu-long Chen, Hui-yong Fan, Samuel Waxmantt, Charles Auffray, Gang Unl, Sai-juan Chen, Zhu Chen, Ii Zhang Ll. |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 5/24/2005, Vol. 102 Issue 21, p7653-7658, 6p |
| مصطلحات موضوعية: |
TRETINOIN, CANCER cells, DERMATOLOGIC agents, CELL cycle, RETINOIDS, BIOLOGICAL rhythms |
| مستخلص: |
Understanding the complexity and dynamics of cancer cells in response to effective therapy requires hypothesis-driven, quantitative, and high-throughput measurement of genes and proteins at both spatial and temporal levels. This study was designed to gain insights into molecular networks underlying the clinical synergy between retinoic acid (RA) and arsenic trioxide (ATO) in acute promyelocytic leukemia (APL), which results in a high-quality disease-free survival in most patients after consolidation with conventional chemotherapy. We have applied an approach inte- grating cDNA micro array, 2D gel electrophoresis with MS, and methods of computational biology to study the effects on APL cell line NM treated with RA, ATO, and the combination of the two agents and collected in a time series. Numerous features were revealed that indicated the coordinated regulation of molecular networks from various aspects of granulocytic differentiation and apoptosis at the transcript and protium levels. These features include an array of transcription factors and cofactors, activation of calcium signaling, stimulation of the IFN pathway, activation of the proteasome system, degradation of the PML-RARE onco protein, restoration of the nuclear body, cell-cycle arrest and gain of apoptotic potential. Hence, this investigation has provided not only a detailed understanding of the combined therapeutic effects of RA/ATO in API but also a road map to approach hematopoietic malignancies at the systems level. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
