التفاصيل البيبلوغرافية
العنوان: |
Crystal structures of the DExH‐box RNA helicase DHX9. |
المؤلفون: |
Lee, Young-Tae, Sickmier, E. Allen, Grigoriu, Simina, Castro, Jennifer, Boriack-Sjodin, P. Ann |
المصدر: |
Acta Crystallographica: Section D, Structural Biology; Nov2023, Vol. 79 Issue 11, p980-991, 12p |
مصطلحات موضوعية: |
RNA helicase, CRYSTAL structure, RNA regulation, DNA helicases, DNA replication, DROSOPHILA |
مستخلص: |
DHX9 is a DExH‐box RNA helicase with versatile functions in transcription, translation, RNA processing and regulation of DNA replication. DHX9 has recently emerged as a promising target for oncology, but to date no mammalian structures have been published. Here, crystal structures of human, dog and cat DHX9 bound to ADP are reported. The three mammalian DHX9 structures share identical structural folds. Additionally, the overall architecture and the individual domain structures of DHX9 are highly conserved with those of MLE, the Drosophila orthologue of DHX9 previously solved in complex with RNA and a transition‐state analogue of ATP. Due to differences in the bound substrates and global domain orientations, the localized loop conformations and occupancy of dsRNA‐binding domain 2 (dsRBD2) differ between the mammalian DHX9 and MLE structures. The combined effects of the structural changes considerably alter the RNA‐binding channel, providing an opportunity to compare active and inactive states of the helicase. Finally, the mammalian DHX9 structures provide a potential tool for structure‐based drug‐design efforts. [ABSTRACT FROM AUTHOR] |
|
Copyright of Acta Crystallographica: Section D, Structural Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
قاعدة البيانات: |
Complementary Index |