التفاصيل البيبلوغرافية
| العنوان: |
v-SNAREs control exocytosis of vesicles from priming to fusion. |
| المؤلفون: |
Borisovska, Maria, Ying Zhao, Tsytsyura, Yaroslav, Glyvuk, Nataliya, Takamori, Shigeo, Matti, Ulf, Rettig, Jens, Südhof, Thomas, Bruns, Dieter |
| المصدر: |
EMBO Journal; 6/15/2005, Vol. 24 Issue 12, p2114-2126, 13p |
| مصطلحات موضوعية: |
EXOCYTOSIS, CELLULAR mechanics, CYTOPLASMIC granules, ORGANELLE formation, BIOMOLECULES, MOLECULAR biology |
| مستخلص: |
SNARE proteins (soluble NSF-attachment protein receptors) are thought to be central components of the exocytotic mechanism in neurosecretory cells, but their precise function remained unclear. Here, we show that each of the vesicle-associated SNARE proteins (v-SNARE) of a chromaffin granule, synaptobrevin II or cellubrevin, is sufficient to support Ca2+-dependent exocytosis and to establish a pool of primed, readily releasable vesicles. In the absence of both proteins, secretion is abolished, without affecting biogenesis or docking of granules indicating that v-SNAREs are absolutely required for granule exocytosis. We find that synaptobrevin II and cellubrevin differentially control the pool of readily releasable vesicles and show that the v-SNARE's amino terminus regulates the vesicle's primed state. We demonstrate that dynamics of fusion pore dilation are regulated by v-SNAREs, indicating their action throughout exocytosis from priming to fusion of vesicles. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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