التفاصيل البيبلوغرافية
| العنوان: |
DAZL Knockout Pigs as Recipients for Spermatogonial Stem Cell Transplantation. |
| المؤلفون: |
Lara, Nathalia L. M., Goldsmith, Taylor, Rodriguez-Villamil, Paula, Ongaratto, Felipe, Solin, Staci, Webster, Dennis, Ganbaatar, Uyanga, Hodgson, Shane, Corbière, Stanislas M. A. S., Bondareva, Alla, Carlson, Daniel F., Dobrinski, Ina |
| المصدر: |
Cells (2073-4409); Nov2023, Vol. 12 Issue 21, p2582, 15p |
| مصطلحات موضوعية: |
STEM cell transplantation, LEYDIG cells, SPERMATOGENESIS, SEMEN, CELL size, SWINE, SEMINIFEROUS tubules, BRAIN death |
| مستخلص: |
Spermatogonial stem cell (SSC) transplantation into the testis of a germ cell (GC)-depleted surrogate allows transmission of donor genotype via donor-derived sperm produced by the recipient. Transplantation of gene-edited SSCs provides an approach to propagate gene-edited large animal models. DAZL is a conserved RNA-binding protein important for GC development, and DAZL knockout (KO) causes defects in GC commitment and differentiation. We characterized DAZL-KO pigs as SSC transplantation recipients. While there were GCs in 1-week-old (wko) KO, complete GC depletion was observed by 10 wko. Donor GCs were transplanted into 18 DAZL-KO recipients at 10–13 wko. At sexual maturity, semen and testes were evaluated for transplantation efficiency and spermatogenesis. Approximately 22% of recipient seminiferous tubules contained GCs, including elongated spermatids and proliferating spermatogonia. The ejaculate of 89% of recipients contained sperm, exclusively from donor origin. However, sperm concentration was lower than the wild-type range. Testicular protein expression and serum hormonal levels were comparable between DAZL-KO and wild-type. Intratesticular testosterone and Leydig cell volume were increased, and Leydig cell number decreased in transplanted DAZL-KO testis compared to wild-type. In summary, DAZL-KO pigs support donor-derived spermatogenesis following SSC transplantation, but low spermatogenic efficiency currently limits their use for the production of offspring. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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