التفاصيل البيبلوغرافية
| العنوان: |
Registration of Functioning of a Single Horseradish Peroxidase Macromolecule with a Solid-State Nanopore. |
| المؤلفون: |
Ivanov, Yuri D., Ableev, Alexander N., Shumov, Ivan D., Ivanova, Irina A., Vaulin, Nikita V., Lebedev, Denis V., Bukatin, Anton S., Mukhin, Ivan S., Archakov, Alexander I. |
| المصدر: |
International Journal of Molecular Sciences; Nov2023, Vol. 24 Issue 21, p15636, 8p |
| مصطلحات موضوعية: |
HORSERADISH peroxidase, SINGLE molecules, MACROMOLECULES, SILICON nitride, HYDROGEN peroxide |
| مستخلص: |
Currently, nanopore-based technology for the determination of the functional activity of single enzyme molecules continues its development. The use of natural nanopores for studying single enzyme molecules is known. At that, the approach utilizing artificial solid-state nanopores is also promising but still understudied. Herein, we demonstrate the use of a nanotechnology-based approach for the investigation of the enzymatic activity of a single molecule of horseradish peroxidase with a solid-state nanopore. The artificial 5 nm solid-state nanopore has been formed in a 40 nm thick silicon nitride structure. A single molecule of HRP has been entrapped into the nanopore. The activity of the horseradish peroxidase (HRP) enzyme molecule inserted in the nanopore has been monitored by recording the time dependence of the ion current through the nanopore in the course of the reaction of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) oxidation reaction. We have found that in the process of ABTS oxidation in the presence of 2.5 mM hydrogen peroxide, individual HRP enzyme molecules are able to retain activity for approximately 700 s before a decrease in the ion current through the nanopore, which can be explained by structural changes of the enzyme. [ABSTRACT FROM AUTHOR] |
|
Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder