التفاصيل البيبلوغرافية
| العنوان: |
A novel chimeric RNA originating from BmCPV S4 and Bombyx mori HDAC11 transcripts regulates virus proliferation. |
| المؤلفون: |
Pan, Jun, Wei, Shulin, Qiu, Qunnan, Tong, Xinyu, Shen, Zeen, Zhu, Min, Hu, Xiaolong, Gong, Chengliang |
| المصدر: |
PLoS Pathogens; 12/4/2023, Vol. 19 Issue 12, p1-19, 19p |
| مصطلحات موضوعية: |
SILKWORMS, RNA replicase, RNA virus infections, RNA viruses, DOUBLE-stranded RNA, RNA, CHIMERIC proteins, REVERSE transcriptase |
| مستخلص: |
Polymerases encoded by segmented negative-strand RNA viruses cleave 5'-m7G-capped host transcripts to prime viral mRNA synthesis ("cap-snatching") to generate chimeric RNA, and trans-splicing occurs between viral and cellular transcripts. Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), an RNA virus belonging to Reoviridae, is a major pathogen of silkworm (B. mori). The genome of BmCPV consists of 10 segmented double-stranded RNAs (S1-S10) from which viral RNAs encoding a protein are transcribed. In this study, chimeric silkworm-BmCPV RNAs, in which the sequence derived from the silkworm transcript could fuse with both the 5' end and the 3' end of viral RNA, were identified in the midgut of BmCPV-infected silkworms by RNA_seq and further confirmed by RT-PCR and Sanger sequencing. A novel chimeric RNA, HDAC11-S4 RNA 4, derived from silkworm histone deacetylase 11 (HDAC11) and the BmCPV S4 transcript encoding viral structural protein 4 (VP4), was selected for validation by in situ hybridization and Northern blotting. Interestingly, our results indicated that HDAC11-S4 RNA 4 was generated in a BmCPV RNA-dependent RNA polymerase (RdRp)-independent manner and could be translated into a truncated BmCPV VP4 with a silkworm HDAC11-derived N-terminal extension. Moreover, it was confirmed that HDAC11-S4 RNA 4 inhibited BmCPV proliferation, decreased the level of H3K9me3 and increased the level of H3K9ac. These results indicated that during infection with BmCPV, a novel mechanism, different from that described in previous reports, allows the genesis of chimeric silkworm-BmCPV RNAs with biological functions. Author summary: It has previously been reported that start codons with cap-snatched host transcripts or trans-splicing can generate chimeric RNAs on ssRNA and dsDNA viruses; however, whether chimeric RNAs can be generated on dsRNA viruses remains a mystery. We identified host-virus chimeric RNAs in a dsRNA virus infection model (BmCPV in silkworm), which is a novel phenomenon. What's more, our results indicated that the selected chimeric RNA HDAC11-S4 RNA 4 (derived from silkworm histone deacetylase 11 (HDAC11) and the BmCPV S4 transcript encoding viral structural protein 4 (VP4)) was generated in a BmCPV RNA-dependent RNA polymerase (RdRp)-independent manner and could be translated into a truncated BmCPV VP4 with a silkworm HDAC11-derived N-terminal extension. Further, We found that the chimeric RNA inhibited virus proliferation and decreased histone methylation, and increased histone acetylation. That is to say, the chimeric protein has activity and provides novel functionality compared to either of the normal versions of the two components on their own. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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