التفاصيل البيبلوغرافية
| العنوان: |
Total wash elimination for solid phase peptide synthesis. |
| المؤلفون: |
Collins, Jonathan M., Singh, Sandeep K., White, Travis A., Cesta, Drew J., Simpson, Colin L., Tubb, Levi J., Houser, Christopher L. |
| المصدر: |
Nature Communications; 12/9/2023, Vol. 14 Issue 1, p1-11, 11p |
| مصطلحات موضوعية: |
PEPTIDE synthesis, WASTE minimization, PEPTIDES, MANUFACTURING processes, AMINO acids |
| مستخلص: |
We present a process for solid phase peptide synthesis (SPPS) that completely eliminates all solvent intensive washing steps during each amino acid addition cycle. A key breakthrough is the removal of a volatile Fmoc deprotection base through bulk evaporation at elevated temperature while preventing condensation on the vessel surfaces with a directed headspace gas flushing. This process was demonstrated at both research and production scales without any impact on product quality and when applied to a variety of challenging sequences (up to 89 amino acids in length). The overall result is an extremely fast, high purity, scalable process with a massive waste reduction (up to 95%) while only requiring 10–15% of the standard amount of base used. This transformation of SPPS represents a step-change in peptide manufacturing process efficiency, and should encourage expanded access to peptide-based therapeutics. Peptide drug manufacturing commonly uses large amounts of hazardous solvents primarily due to multiple washings after each step. Here, the authors introduce the concept of a completely wash-free methodology that enables up to 95% waste reduction. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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