التفاصيل البيبلوغرافية
| العنوان: |
Profiling ubiquitin signalling with UBIMAX reveals DNA damage- and SCFβ-Trcp1-dependent ubiquitylation of the actin-organizing protein Dbn1. |
| المؤلفون: |
Colding-Christensen, Camilla S., Kakulidis, Ellen S., Arroyo-Gomez, Javier, Hendriks, Ivo A., Arkinson, Connor, Fábián, Zita, Gambus, Agnieszka, Mailand, Niels, Duxin, Julien P., Nielsen, Michael L. |
| المصدر: |
Nature Communications; 12/14/2023, Vol. 14 Issue 1, p1-19, 19p |
| مصطلحات موضوعية: |
UBIQUITINATION, DNA repair, UBIQUITIN, DOUBLE-strand DNA breaks, XENOPUS eggs, UBIQUITIN ligases |
| مستخلص: |
Ubiquitin widely modifies proteins, thereby regulating most cellular functions. The complexity of ubiquitin signalling necessitates unbiased methods enabling global detection of dynamic protein ubiquitylation. Here, we describe UBIMAX (UBiquitin target Identification by Mass spectrometry in Xenopus egg extracts), which enriches ubiquitin-conjugated proteins and quantifies regulation of protein ubiquitylation under precise and adaptable conditions. We benchmark UBIMAX by investigating DNA double-strand break-responsive ubiquitylation events, identifying previously known targets and revealing the actin-organizing protein Dbn1 as a major target of DNA damage-induced ubiquitylation. We find that Dbn1 is targeted for proteasomal degradation by the SCFβ-Trcp1 ubiquitin ligase, in a conserved mechanism driven by ATM-mediated phosphorylation of a previously uncharacterized β-Trcp1 degron containing an SQ motif. We further show that this degron is sufficient to induce DNA damage-dependent protein degradation of a model substrate. Collectively, we demonstrate UBIMAX's ability to identify targets of stimulus-regulated ubiquitylation and reveal an SCFβ-Trcp1-mediated ubiquitylation mechanism controlled directly by the apical DNA damage response kinases. Using Xenopus egg extracts, the authors developed a mass spectrometry method (UBIMAX) to identify proteins ubiquitylated in response to defined DNA lesions. Highlighting UBIMAX's versatility, they describe the ubiquitylation of the actin regulator Dbn1 in response to DNA double-strand breaks. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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