التفاصيل البيبلوغرافية
| العنوان: |
糙皮侧耳HMG-box转录因子分析与候选基因克隆表达. (Chinese) |
| Alternate Title: |
Analysis of HMG-box Transcription Factors in Pleurotus ostreatus and Cloning of a Candidate Gene. (English) |
| المؤلفون: |
赵 琛, 王 翔, 闫 舟, 郝金斌, 王 芳, 刘靖宇 |
| المصدر: |
Acta Edulis Fungi; 2023, Vol. 30 Issue 6, p1-11, 11p |
| Abstract (English): |
Fourteen HMG-box protein coding genes, PoHMG1-PoHMG14, were identified in the whole genome of Pleurotus ostreatus, and then analyzed for their characteristics, structure and phylogeny. The results showed that the tertiary structures of these HMG-box transcription factors were highly similar. Their relative molecular weight ranged from 6 893.79 to 60 397.29 Da. PoHMG6, PoHMG11, PoHMG12 and PoHMG13 were phylogenetically closely related. Based on the transcriptome data of P. ostreatus in mycelium, primordium, young fruiting body and mature fruiting body, PoHMG11 was cloned and analyzed for its function and expression at different growth stages.The full-length of PoHMG11 was found to be 1 517 bp, which encodes 489 amino acid residues and contains a HMG-box domain. PoHMG11 was heterologously expressed in E. coli to yield a protein product of 5.5×104, and the highest expression level was achieved when the recombinant protein was induced by 1 mol· L-1 IPTG at 25 ℃ for 6 h. Real-time quantitative PCR analysis showed that the expression level of PoHMG11 was significantly increased by more than five times in mature fruiting bodies compared with other stages, suggesting that the gene may be involved in regulating fruiting body maturation of P. ostreatus. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
在糙皮侧耳（Pleurotus ostreatus）的全基因组中鉴定出14个高速泳动蛋白（high-mobility group box,HMG-box）的编码基因（PoHMG1~PoHMG14）,对其进行特征、结构与系统进化分析,发现所有的HMG-box转录因子蛋白的三级结构高度相似,相对分子质量为6 893.79~60 397.29, 蛋白PoHMG6、PoHMG11、PoHMG12与PoHMG13之间的亲缘关系较近。基于糙皮侧耳在菌丝、原基、幼嫩子实体和成熟子实体时期的转录组数据,对筛选到的转录因子基因PoHMG11进行克隆分析并探究其功能及表达特性,发现该基因全长序列1 517 bp,编码489个氨基酸,含有一个HMG-box结构域。通过大肠杆菌原核表达载体体外诱导表达出相对分子质量为5.5×104的蛋白,该重组蛋白在 1 mol·L-1 IPTG、25 ℃诱导表达6 h 时表达量最高。利用实时荧光定量PCR（qRT-PCR）分析糙皮侧耳在菌丝、原基、幼嫩子实体和成熟子实体阶段PoHMG11基因的相对表达量,发现该基因在成熟糙皮侧耳子实体中的表达量比其他生长发育时期显著升高5倍以上,推测该基因可能参与调控糙皮侧耳子实体成熟过程。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
