التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of DiaSorin Liaison® calprotectin fecal assay adapted for pleural effusion. |
| المؤلفون: |
de Paz Poves, Cristina, Barneo-Caragol, Clara, Cillero Sánchez, Ana Isabel, Jimenez Mendiguchia, Lucía, Quirós Caso, Covadonga, Moreno Rodríguez, María, López González, Francisco J., Prieto García, Mᵃ Belén |
| المصدر: |
Advances in Laboratory Medicine / Avances en Medicina de Laboratorio; Dec2023, Vol. 4 Issue 4, p419-424, 6p |
| مصطلحات موضوعية: |
ZINC metabolism, FECAL analysis, PLEURAL effusions, PLEURA, AUTOANALYZERS, IMMUNOASSAY, QUALITY assurance, RESEARCH funding, DESCRIPTIVE statistics, AUTOMATION, CALCIUM-binding proteins, SENSITIVITY & specificity (Statistics), CARRIER proteins |
| مستخلص: |
Calprotectin (CP) is a calcium and zinc binding protein that is widely measured on faecal samples but its determination in other biological fluids might be of interest. The aim of this work was to validate the measurement of CP in pleural fluid by chemiluminescence. LIAISON®XL, a fully automated chemiluminescence analyzer, was used for CP quantification on pleural fluid. A validation protocol was designed using both quality control materials provided by the manufacturer and pools of pleural fluid samples. Stability, imprecision, bias, linearity, detection capability and carry over effect were evaluated. CP was stable on pleural fluid at least one week, under refrigerated conditions, and four weeks at −80 °C. The observed intra- and inter-day imprecision was between 2.2 and 6.49 %, with a negative bias under 5.51 %. The linearity of the method was verified up to 2,000 ng/mL. The LoQ for the assay was 48.52 ng/mL. A statistically significant carry-over effect was observed after measuring CP concentrations above the upper limit of linearity, but given the observed magnitude, a clinically relevant impact should not be expected. DiaSorin Liaison® calprotectin assay allows reliable measurement of CP in pleural fluid. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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