التفاصيل البيبلوغرافية
| العنوان: |
Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes. |
| المؤلفون: |
Lukšíková, Karolína, Pavlica, Tomáš, Altmanová, Marie, Štundlová, Jana, Pelikánová, Šárka, Simanovsky, Sergey A., Krysanov, Eugene Yu., Jankásek, Marek, Hiřman, Matyáš, Reichard, Martin, Ráb, Petr, Sember, Alexandr |
| المصدر: |
Journal of Fish Biology; Dec2023, Vol. 103 Issue 6, p1501-1514, 14p |
| مصطلحات موضوعية: |
KARYOTYPES, Y chromosome, SATELLITE DNA, SEX chromosomes, KILLIFISHES, CHROMOSOME polymorphism, FLUORESCENCE in situ hybridization, AFRICAN swine fever |
| مستخلص: |
Using African annual killifishes of the genus Nothobranchius from temporary savannah pools with rapid karyotype and sex chromosome evolution, we analysed the chromosomal distribution of telomeric (TTAGGG)n repeat and Nfu‐SatC satellite DNA (satDNA; isolated from Nothobranchius furzeri) in 15 species across the Nothobranchius killifish phylogeny, and with Fundulosoma thierryi as an out‐group. Our fluorescence in situ hybridization experiments revealed that all analysed taxa share the presence of Nfu‐SatC repeat but with diverse organization and distribution on chromosomes. Nfu‐SatC landscape was similar in conspecific populations of Nothobranchius guentheri and Nothobranchius melanospilus but slightly‐to‐moderately differed between populations of Nothobranchius pienaari, and between closely related Nothobranchius kuhntae and Nothobranchius orthonotus. Inter‐individual variability in Nfu‐SatC patterns was found in N. orthonotus and Nothobranchius krysanovi. We revealed mostly no sex‐linked patterns of studied repetitive DNA distribution. Only in Nothobranchius brieni, possessing multiple sex chromosomes, Nfu‐SatC repeat occupied a substantial portion of the neo‐Y chromosome, similarly as formerly found in the XY sex chromosome system of turquoise killifish N. furzeri and its sister species Nothobranchius kadleci—representatives not closely related to N. brieni. All studied species further shared patterns of expected telomeric repeats at the ends of all chromosomes and no additional interstitial telomeric sites. In summary, we revealed (i) the presence of conserved satDNA class in Nothobranchius clades (a rare pattern among ray‐finned fishes); (ii) independent trajectories of Nothobranchius sex chromosome differentiation, with recurrent and convergent accumulation of Nfu‐SatC on the Y chromosome in some species; and (iii) genus‐wide shared tendency to loss of telomeric repeats during interchromosomal rearrangements. Collectively, our findings advance our understanding of genome structure, mechanisms of karyotype reshuffling, and sex chromosome differentiation in Nothobranchius killifishes from the genus‐wide perspective. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Fish Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder