Chemotaxonomical study of two actinomycetes strains ID03-700 and ID03-721.

التفاصيل البيبلوغرافية
العنوان: Chemotaxonomical study of two actinomycetes strains ID03-700 and ID03-721.
المؤلفون: Ratnakomala, Shanti, Lisdiyanti, Puspita, Otoguro, Misa
المصدر: AIP Conference Proceedings; 2023, Vol. 2972 Issue 1, p1-10, 10p
مصطلحات موضوعية: ACTINOBACTERIA, FOREST litter, BOTANICAL gardens, VITAMIN K2, FATTY acids, STREPTOMYCES
مستخلص: Two actinomycete strains, ID03-700 and ID03-721, isolated from leaf litter samples in the Ekakarya Botanical Garden of Bali using Rehydration and Centrifugation methods, were observed for their growth, morphological, physiological, and chemotaxonomic characteristics. Both isolates are Gram-positive, aerobic, and have branched hyphae. ID03-700 and ID03-721 strains grew well in yeast extract-malt agar (ISP-2) and showed orange colonies. The chemotaxonomic and morphological characterization showed that the two isolates belong to a cluster of the genus Actinoplanes, which has meso−diaminopimelic acid, menaquinone MK-9(H4), phospholipid type PII, and iso-C16:0, iso-C15:0, anteiso-C15:0 as a major fatty acid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates closely related to the genus Actinoplanes. Further, a phylogenetic tree of the family Micromonosporaceae revealed that the ID03-700 and ID03-721 strains had similarities with Actinoplanes durhamensis strain JCM 7625T and Actinoplanes ferrugineus strain JCM 3277T, respectively, with a similarity value of 97.69 %. According to the findings, the two isolates are promising as two novel bacterial strains. [ABSTRACT FROM AUTHOR]
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قاعدة البيانات: Complementary Index
الوصف
تدمد:0094243X
DOI:10.1063/5.0183406