التفاصيل البيبلوغرافية
| العنوان: |
Gelatin methacryloyl hydrogel with and without dental pulp stem cells for TMJ regeneration: An in vivo study in rabbits. |
| المؤلفون: |
Monteiro, Joao Luiz, Takusagawa, Toru, Sampaio, Gerhilde C., He, Helen, de Oliveira e Silva, Emanuel D., Vasconcelos, Belmiro C. E., McCain, Joseph P., Redmond, Robert W., Randolph, Mark A., Guastaldi, Fernando P. S. |
| المصدر: |
Journal of Oral Rehabilitation; Feb2024, Vol. 51 Issue 2, p394-403, 10p |
| مصطلحات موضوعية: |
STEM cell transplantation, TEMPOROMANDIBULAR joint surgery, THERAPEUTIC use of proteins, BIOLOGICAL models, EXPERIMENTAL design, CARTILAGE, IN vivo studies, HYDROCOLLOID surgical dressings, ANIMAL experimentation, CONNECTIVE tissues, INFLAMMATION, RABBITS, DENTAL pulp, TISSUE engineering, DESCRIPTIVE statistics, ARTICULAR cartilage injuries, BONE regeneration, BONE density, COMPUTED tomography |
| مستخلص: |
Background: In the last decade, tissue‐engineering strategies for regenerating the temporomandibular joint (TMJ) have been investigated. This may be a promising strategy for the minimally invasive restoration of joint integrity. Objectives: To evaluate whether dental pulp stem cells (DPSCs) loaded in a light‐occured hydrogel made of gelatin methacryloyl (GelMA) enhance the regeneration of osteochondral defects in the rabbit TMJ. Materials and Methods: Defects were filled with GelMA alone (control group; n = 4) or filled with GelMA loaded with rabbit DPSCs (experimental group; n = 4), In one group, the TMJ capsule was opened without creating a defect (sham group; n = 2). The following micro‐CT parameters were analysed: bone volume to total volume ratio (BV/TV%) and bone mineral density (BMD). Histological evaluation was performed to assess cartilage regeneration features. A semi‐quantitative scoring system was also used to evaluate the defects. Results: All groups had no statistical difference regarding the micro‐CT parameters. The highest mean healing score was found for the experimental group. After 4 weeks, there were no signs of hydrogel in either group or no signs of inflammation in the adjacent tissues. The tissue formed in the defect was dense fibrous connective tissue. Conclusion: Adding DPSCs to GelMA did not provide a regenerative enhancement in TMJ osteochondral defects. This resulted in similar micro‐CT parameters after 4 weeks of healing, with improved signs of subchondral bone regeneration but no cartilage regeneration. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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