التفاصيل البيبلوغرافية
| العنوان: |
Histidine transport is essential for the growth of Staphylococcus aureus at low pH. |
| المؤلفون: |
Beetham, Catrin M., Schuster, Christopher F., Kviatkovski, Igor, Santiago, Marina, Walker, Suzanne, Gründling, Angelika |
| المصدر: |
PLoS Pathogens; 1/16/2024, Vol. 20 Issue 1, p1-29, 29p |
| مصطلحات موضوعية: |
HISTIDINE, GENE expression, GENETIC code, HUMAN ecology, STAPHYLOCOCCUS aureus, CELL physiology |
| مستخلص: |
Staphylococcus aureus is an opportunistic pathogen capable of causing many different human diseases. During colonization and infection, S. aureus will encounter a range of hostile environments, including acidic conditions such as those found on the skin and within macrophages. However, little is known about the mechanisms that S. aureus uses to detect and respond to low pH. Here, we employed a transposon sequencing approach to determine on a genome-wide level the genes required or detrimental for growth at low pH. We identified 31 genes that were essential for the growth of S. aureus at pH 4.5 and confirmed the importance of many of them through follow up experiments using mutant strains inactivated for individual genes. Most of the genes identified code for proteins with functions in cell wall assembly and maintenance. These data suggest that the cell wall has a more important role than previously appreciated in promoting bacterial survival when under acid stress. We also identified several novel processes previously not linked to the acid stress response in S. aureus. These include aerobic respiration and histidine transport, the latter by showing that one of the most important genes, SAUSA300_0846, codes for a previously uncharacterized histidine transporter. We further show that under acid stress, the expression of the histidine transporter gene is increased in WT S. aureus. In a S. aureus SAUSA300_0846 mutant strain expression of the histidine biosynthesis genes is induced under acid stress conditions allowing the bacteria to maintain cytosolic histidine levels. This strain is, however, unable to maintain its cytosolic pH to the same extent as a WT strain, revealing an important function specifically for histidine transport in the acid stress response of S. aureus. Author summary: Staphylococcus aureus is an important human bacterial pathogen that can cause a range of diseases. During infection, the pathogen will encounter a hostile environment within the human host, including acidic conditions such as those found on the skin and within macrophages. The bacterium has developed sophisticated strategies to survive and grow under such harsh conditions. Here we performed a genome wide screen to identify factors that are required by this pathogen to survive under acid stress conditions and identified several novel processes including histidine uptake. Understanding the response of S. aureus to deal with acid stress conditions will help us better manage infections. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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