التفاصيل البيبلوغرافية
| العنوان: |
COMPARATIVE EVALUATION OF BLOOD SMEAR EXAMINATION AND POLYMERASE CHAIN REACTION FOR DIAGNOSIS OF CANINE BABESIOSIS. |
| المؤلفون: |
Mukartal, S. Y., Malatesh, D. S., Sreedhara, J. N., Kharate, Arun, Patil, Shivanagouda |
| المصدر: |
Journal of Experimental Zoology India; Jan2024, Vol. 27 Issue 1, p1247-1250, 4p |
| مصطلحات موضوعية: |
BLOOD testing, POLYMERASE chain reaction, BABESIOSIS, DOG adoption, PROTOZOAN diseases, TICK infestations |
| مستخلص: |
Canine babesiosis is a tick-borne, protozoan disease caused by intraerythrocytic parasites Babesia canis and B. gibsoni. The present study was undertaken to evaluate the conventional method of blood smear examination and molecular method PCR (polymerase chain reaction) as diagnostic tools for detection of canine babesiosis. Dogs (>70.0%) exhibited clinical symptoms of high fever (104°F), anaemia, lethargy, history of tick infestation, congested/pale/icteric mucous membranes, hematuria and epistaxis. A total of 102 blood samples from dogs suspected for canine babesiosis were collected from different breeds, gender and age group of animals presented to Veterinary College Hospital, Hebbal, Bengaluru. Of 102 samples screened, 40 (39.21%) and 66 (64.70%) were positive by blood microscopy and PCR, respectively. Overall prevalence of canine babesiosis was 31 (30.39%) and 50 (49.02%) with B. canis and B. gibsoni, respectively. Mixed infections with both B. canis and B. gibsoni were detected in 25 (24.50%) samples. Prevalence of Canine babesiosis was moderately high in dogs and adoption of combinations of conventional method and molecular method is recommended with microscopy as a screening test and PCR as the confirmatory test. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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