التفاصيل البيبلوغرافية
| العنوان: |
Tubb4b is required for multi-ciliogenesis in the mouse. |
| المؤلفون: |
Sewell, Mycah T., Legué, Emilie, Liem Jr, Karel F. |
| المصدر: |
Development (09501991); Jan2024, Vol. 151 Issue 1, p1-7, 7p |
| مصطلحات موضوعية: |
CILIA & ciliary motion, TUBULINS, POST-translational modification, MICE, AXONEMES, MICROTUBULES, ORGANELLES |
| مستخلص: |
Cilia are microtubule (MT)-based organelles present on the surface of nearly all vertebrate cells. MTs are polymers of a- and ß-tubulins that are each encoded by multiple, individual isotype genes. Tubulin isotype composition is thought to influence MT behaviors. Ciliary MTs differ from other MTs in the cell in terms of organization, stability and post-translational modifications. However, little is known about the tubulin isotypes that build ciliary MTs and the functional requirements for tubulin isotypes in cilia have not been examined in vertebrates. Here, we have tested the role of the ß-tubulin isotype genes in the mouse that harbor a conserved amino acid motif associated with ciliated organisms. We found that Tubb4b localizes to cilia in multiciliated cells (MCCs) specifically. In respiratory and oviduct MCCs, Tubb4b is asymmetrically localized within multi-cilia, indicating that the tubulin isotype composition changes along the length of the ciliary axonemal MTs. Deletion of Tubb4b resulted in striking structural defects within the axonemes of multi-cilia, without affecting primary cilia. These studies show that Tubb4b is essential for the formation of a specific MT-based subcellular organelle and sheds light on the requirements of tubulin isotypes in cilia. [ABSTRACT FROM AUTHOR] |
|
Copyright of Development (09501991) is the property of Company of Biologists Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
